FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2074464730> ?p ?o ?g. }

• W2074464730 endingPage "315" @default.
• W2074464730 startingPage "310" @default.
• W2074464730 abstract "Polyclonal antibodies were prepared against synthetic peptides corresponding to four different extramembrane segments of the rat glucagon receptor. The antibodies bound specifically to native glucagon receptor as judged by immunofluorescence microscopy of cultured cells expressing a synthetic gene for the receptor. Antibodies to peptides designated PR-15 and DK-12 were directed against amino acid residues 103-117 and 126-137, respectively, of the extracellular N-terminal tail. Antibody to peptide KD-14 was directed against residues 206-219 of the first extracellular loop, and antibody to peptide ST-18, against the intracellular C-terminal tail, residues 468-485. The DK-12 and KD-14 antibodies, but not the PR-15 and ST-18 antibodies, could effectively block binding of 125I-labeled glucagon to its receptor in liver membranes. Incubation of these antibodies with rat liver membranes resulted in both a decrease in the maximal hormonal binding capacity and an apparent decrease in glucagon affinity for its receptor. These effects were abolished in the presence of excess specific peptide antigen. In addition, DK-12 and KD-14 antibodies, but not PR-15 and ST-18 antibodies, interfered with glucagon-induced adenylyl cyclase activation in rat liver membranes and behaved as functional glucagon antagonists. These results demonstrate that DK-12 and KD-14 antibodies are pharmacologically active glucagon antagonists and strongly suggest that residues 126-137 of the N-terminal tail and residues 206-219 of the first extracellular loop contain determinants of ligand binding and may comprise the primary ligand-binding site on the glucagon receptor." @default.
• W2074464730 created "2016-06-24" @default.
• W2074464730 creator A5015924061 @default.
• W2074464730 creator A5018415215 @default.
• W2074464730 creator A5047415387 @default.
• W2074464730 creator A5067028169 @default.
• W2074464730 creator A5068083621 @default.
• W2074464730 creator A5076220039 @default.
• W2074464730 date "1996-01-09" @default.
• W2074464730 modified "2023-09-23" @default.
• W2074464730 title "Antibodies against specific extracellular epitopes of the glucagon receptor block glucagon binding." @default.
• W2074464730 cites W1499460529 @default.
• W2074464730 cites W1499968412 @default.
• W2074464730 cites W1510475440 @default.
• W2074464730 cites W1526839960 @default.
• W2074464730 cites W1534834860 @default.
• W2074464730 cites W1577863238 @default.
• W2074464730 cites W1581400573 @default.
• W2074464730 cites W1970614604 @default.
• W2074464730 cites W1978958492 @default.
• W2074464730 cites W2005097235 @default.
• W2074464730 cites W2030264781 @default.
• W2074464730 cites W2031423487 @default.
• W2074464730 cites W2039911916 @default.
• W2074464730 cites W2045543114 @default.
• W2074464730 cites W2052973557 @default.
• W2074464730 cites W2053418558 @default.
• W2074464730 cites W2057971042 @default.
• W2074464730 cites W2074501705 @default.
• W2074464730 cites W2080464419 @default.
• W2074464730 cites W2082203652 @default.
• W2074464730 cites W2084654327 @default.
• W2074464730 cites W2140998261 @default.
• W2074464730 cites W2160088320 @default.
• W2074464730 cites W2396394505 @default.
• W2074464730 doi "https://doi.org/10.1073/pnas.93.1.310" @default.
• W2074464730 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/40228" @default.
• W2074464730 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8552628" @default.
• W2074464730 hasPublicationYear "1996" @default.
• W2074464730 type Work @default.
• W2074464730 sameAs 2074464730 @default.
• W2074464730 citedByCount "49" @default.
• W2074464730 countsByYear W20744647302012 @default.
• W2074464730 countsByYear W20744647302014 @default.
• W2074464730 countsByYear W20744647302016 @default.
• W2074464730 countsByYear W20744647302021 @default.
• W2074464730 crossrefType "journal-article" @default.
• W2074464730 hasAuthorship W2074464730A5015924061 @default.
• W2074464730 hasAuthorship W2074464730A5018415215 @default.
• W2074464730 hasAuthorship W2074464730A5047415387 @default.
• W2074464730 hasAuthorship W2074464730A5067028169 @default.
• W2074464730 hasAuthorship W2074464730A5068083621 @default.
• W2074464730 hasAuthorship W2074464730A5076220039 @default.
• W2074464730 hasBestOaLocation W20744647301 @default.
• W2074464730 hasConcept C153911025 @default.
• W2074464730 hasConcept C159654299 @default.
• W2074464730 hasConcept C170493617 @default.
• W2074464730 hasConcept C185280430 @default.
• W2074464730 hasConcept C185592680 @default.
• W2074464730 hasConcept C195616568 @default.
• W2074464730 hasConcept C203014093 @default.
• W2074464730 hasConcept C2778542361 @default.
• W2074464730 hasConcept C2778563252 @default.
• W2074464730 hasConcept C2778938600 @default.
• W2074464730 hasConcept C28406088 @default.
• W2074464730 hasConcept C55493867 @default.
• W2074464730 hasConcept C71315377 @default.
• W2074464730 hasConcept C86803240 @default.
• W2074464730 hasConcept C956191 @default.
• W2074464730 hasConceptScore W2074464730C153911025 @default.
• W2074464730 hasConceptScore W2074464730C159654299 @default.
• W2074464730 hasConceptScore W2074464730C170493617 @default.
• W2074464730 hasConceptScore W2074464730C185280430 @default.
• W2074464730 hasConceptScore W2074464730C185592680 @default.
• W2074464730 hasConceptScore W2074464730C195616568 @default.
• W2074464730 hasConceptScore W2074464730C203014093 @default.
• W2074464730 hasConceptScore W2074464730C2778542361 @default.
• W2074464730 hasConceptScore W2074464730C2778563252 @default.
• W2074464730 hasConceptScore W2074464730C2778938600 @default.
• W2074464730 hasConceptScore W2074464730C28406088 @default.
• W2074464730 hasConceptScore W2074464730C55493867 @default.
• W2074464730 hasConceptScore W2074464730C71315377 @default.
• W2074464730 hasConceptScore W2074464730C86803240 @default.
• W2074464730 hasConceptScore W2074464730C956191 @default.
• W2074464730 hasIssue "1" @default.
• W2074464730 hasLocation W20744647301 @default.
• W2074464730 hasLocation W20744647302 @default.
• W2074464730 hasLocation W20744647303 @default.
• W2074464730 hasLocation W20744647304 @default.
• W2074464730 hasOpenAccess W2074464730 @default.
• W2074464730 hasPrimaryLocation W20744647301 @default.
• W2074464730 hasRelatedWork W1975648827 @default.
• W2074464730 hasRelatedWork W1983657734 @default.
• W2074464730 hasRelatedWork W2009181770 @default.
• W2074464730 hasRelatedWork W2056973121 @default.
• W2074464730 hasRelatedWork W2061888378 @default.
• W2074464730 hasRelatedWork W2078543303 @default.
• W2074464730 hasRelatedWork W2117726724 @default.

• next