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- W2074524934 abstract "A novel three-dimensional, magnetic and electroactive nanoprobes were constructed for the first time. Using hemin (TPP) as electron mediator, mutli-walled carbon nanotubes with carboxyl groups (MCNTs) as supporter, a novel (MCNTs-TPP-Fe 3 O 4 ) magnetic nanocomposites were first prepared. Then using alpha-fetoprotein (AFP) as model system, gold nanoparticles (Au NPs) as immobilizing matrix, the AFP/anti-AFP/Au NPs/ MCNTs-TPP-Fe 3 O 4 nanoprobes were prepared and then dropped on the surface of screen-printed working electrode (SPCE) to construct a new amperometric immunosensors for detecting biomakers. The microstructure of different nanoparticles were observed by transmission electron microscopy (TEM) and X -ray fluorescence spectrometery (XRFS). Under optimal experimental conditions, the logarithm of response signal was proportional to the logarithm of AFP concentration from 0.1 to 200 ng/mL, with a correlation coefficient of 0.998. The detection limit was 0.04 ng/mL at a signal-to-noise ratio of 3. The proposed method offered a platform for fast, sensitive and simultaneous determination for serum samples." @default.
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- W2074524934 date "2011-09-01" @default.
- W2074524934 modified "2023-09-27" @default.
- W2074524934 title "A Novel Amperometric Immunosensor for Detection of Alpha-Fetoprotein Based on Magnetic and Electroactive Nanoprobes" @default.
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- W2074524934 doi "https://doi.org/10.4028/www.scientific.net/amr.345.411" @default.
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