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- W2074536670 abstract "Candida utilis can grow efficiently in a simple synthetic medium at low pH. The electron donor for yeast-mediated enantioselective bioreduction of acetophenone was ethanol, which was also used to dissolve the acetophenone to make a successful semi-fed-batch type reaction. NADH was added externally to assist the reduction. The pH of the culture was varied from 2·3 to 6·0 and the bioconversion temperature was controlled at 30°C. In general, the productivity of yeast (PN) for the production of 1-phenylethanol was increased by raising the pH. The maximum PN was 4·17 mmol kg−1 h−1 at pH 6·0. However, the largest enantiomer excess (ee value) for the synthesis of S-(−)-1-phenylethanol was 94·8 ± 0·8% with the pH controlled at 5·0." @default.
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- W2074536670 date "1996-01-01" @default.
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- W2074536670 title "Enantioselective synthesis of S-(−)-1-phenylethanol in Candida utilis semi-fed-batch cultures" @default.
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- W2074536670 doi "https://doi.org/10.1016/0032-9592(94)00022-0" @default.
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