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• W2074560124 abstract "Immunization of rabbits with tetanus toxoid stimulated the production of an antibody capable of senstitizing homologous skin for anphylactic reactions. These skin reactions were suppressed by antihistamines but not by a serotonin antagonist. The skin sensitizing (homocytotropic) antibody was detectable in serum over a period of 6 to 133 days after a primary injection of tetanus toxoid. an anamnestic response was also observed. The homocytotropic antibody was differentiated from previously established classes of rabbit immunoglobulins by physicochemical means and by immunological methods using specific antisera. In passive transfer experiments it was found to persist in rabbit skin for at least 6 weeks. The antibody was excreted in colostrum of immunized rabbits and was probably transmitted to the fetus in utero. IgA isolated from colostrum of immunized rabbits was devoid of antibody activities. A molecular weight of approximately 200,000 was estimated for the homocytropic antibody on the basis of its elution pattern in Sephadex G 200 gel filtration. The antibody was heat stable, and was susceptible to sulfhydryl reagents. However, the skin sensitizing activity could be recovered in preparations reduced with dithiothreitol (without subsequent alkylation) if the preparations were left with the reducing agent in the cold for 18 hr." @default.
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• W2074560124 creator A5078308821 @default.
• W2074560124 date "1968-11-01" @default.
• W2074560124 modified "2023-09-25" @default.
• W2074560124 title "A unique class of rabbit immunoglobulins eliciting passive cutaneous anaphylaxis in homologous skin" @default.
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• W2074560124 doi "https://doi.org/10.1016/0019-2791(68)90089-x" @default.
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