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- W2074562128 abstract "transparent spheres (do not confuse with yolk granules which are smaller and more opaque). Some 5-10 nuclei are taken up and then injected into recipient eggs in twos or threes. After some practice, it may be possible to inject only 1 nucleus. The same donor egg can serve as a source of nuclei for several injections if one picks nuclei each time from an area which was not injured. After injections are done, the donor eggs should be removed from the slide to avoid confusion. Transplantation of pole cells. Recipient eggs should be at syncytial blastoderm stages (stages 11-135). Slight drying will retract the ooplasm and leave a free space near the pole cells. The eggs are oriented parallel to the direction of the pipette. The best source of donor pole cells is the late blastoderm stage. The egg should be oriented at a wide angle to the pipette. One pierces the egg on the side and works the needle to the base of the pole cells by pushing and pulling (fig. 5). The ceils are aspirated, the pipette withdrawn and inserted into the posterior pole of the recipient egg. Since the needle usually penetrates deeply into the egg after piercing the vitelline membrane, it has to be retracted to bring the opening just under the membrane. If the egg has been properly dried, it will accept all the cells injected. Post-operative care is as for other injections." @default.
- W2074562128 created "2016-06-24" @default.
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- W2074562128 date "1981-12-01" @default.
- W2074562128 modified "2023-10-06" @default.
- W2074562128 title "Improving laboratory safety: replacing benzene by toluene in phosphate analyses" @default.
- W2074562128 cites W1970902024 @default.
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- W2074562128 doi "https://doi.org/10.1007/bf01948409" @default.
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