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- W2074564768 abstract "Abstract Calf fetuin is a well-known glycoprotein found only in the fetus. A similar protein exists in fetal lamb serum but it had been demonstrated only by immunological methods. In the present investigation, we attempted the isolation and characterization of this protein. 1. 1. Fetal lamb serum was analysed by several electrophoretical methods. Its relative complexity does not allow the unambiguous identification of lamb fetuin. 2. 2. Using the alcohol-metal ion fractionation procedure of Spiro, it was possible to isolate a glycoprotein very similar to calf fetuin but in lower yields. Some contaminant proteins were observed. 3. 3. An alternative method is proposed (chromatography on CM-cellulose, pH 4.6, I = 0.01, followed by gel filtration on Sephadex G-200) leading to higher yields of a purer product. 4. 4. Some physicochemical parameters have been measured. E2781% = 4.05, s20,w0 = 3.50 ± 0.10; pHi = 3.85, 4.05, 4.10, 4.40 for the main variants. The apparent molecular weight measured by gel filtration is very much the same as for calf fetuin. 5. 5. The amino acid analyses revealed the presence of 364 residues per mole, representing approximately 79.8% of the weight of the protein. No methionine could be detected, nor any free -SH group, suggesting the presence of 6 disulfide bonds. 6. 6. The N- and C-terminal residues were both isoleucine. 7. 7. The carbohydrate content (approximately 22.75%) was determined by classical methods. Sialic acid (8.50%), is essentially N-acetylneuraminic acid. Hexosamines (6.75%) are glucosamine and galactosamine in the ratio 8:1. Hexoses are galactose (4.50%) and mannose (3.00%). 8. 8. The molecular weight calculated from chemical composition is 48 500. 9. 9. Calf and lamb fetuin appear to be very similar." @default.
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- W2074564768 date "1973-04-01" @default.
- W2074564768 modified "2023-09-27" @default.
- W2074564768 title "Preparation, constantes physiques et constitution chimique de la fetuine d'agneau" @default.
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- W2074564768 doi "https://doi.org/10.1016/0005-2795(73)90366-8" @default.
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