Matches in SemOpenAlex for { <https://semopenalex.org/work/W2074574257> ?p ?o ?g. }
- W2074574257 endingPage "287" @default.
- W2074574257 startingPage "281" @default.
- W2074574257 abstract "Abstract Zebrafish have become an invaluable vertebrate animal model to interrogate small molecule libraries for modulators of complex biological pathways and phenotypes. We have recently described the implementation of a quantitative, high‐content imaging assay in multi‐well plates to analyze the effects of small molecules on Fibroblast Growth Factor (FGF) signaling in vivo. Here we have evaluated the capability of the assay to identify compounds that hyperactivate FGF signaling from a test cassette of agents with known biological activities. Using a transgenic zebrafish reporter line for FGF activity, we screened 1040 compounds from an annotated library of known bioactive agents, including FDA‐approved drugs. The assay identified two molecules, 8‐hydroxyquinoline sulfate and pyrithione zinc, that enhanced FGF signaling in specific areas of the brain. Subsequent studies revealed that both compounds specifically expanded FGF target gene expression. Furthermore, treatment of early stage embryos with either compound resulted in dorsalized phenotypes characteristic of hyperactivation of FGF signaling in early development. Documented activities for both agents included activation of extracellular signal‐related kinase (ERK), consistent with FGF hyperactivation. To conclude, we demonstrate the power of automated quantitative high‐content imaging to identify small molecule modulators of FGF. Birth Defects Research (Part C) 93:281–287, 2011. © 2011 Wiley‐Liss, Inc." @default.
- W2074574257 created "2016-06-24" @default.
- W2074574257 creator A5011676332 @default.
- W2074574257 creator A5045341517 @default.
- W2074574257 creator A5066935152 @default.
- W2074574257 creator A5079722603 @default.
- W2074574257 creator A5087527556 @default.
- W2074574257 date "2011-09-01" @default.
- W2074574257 modified "2023-10-13" @default.
- W2074574257 title "A high-content screening assay in transgenic zebrafish identifies two novel activators of fgf signaling" @default.
- W2074574257 cites W1554593075 @default.
- W2074574257 cites W1811188705 @default.
- W2074574257 cites W1825722006 @default.
- W2074574257 cites W1969441357 @default.
- W2074574257 cites W1980976351 @default.
- W2074574257 cites W1988454868 @default.
- W2074574257 cites W1994279105 @default.
- W2074574257 cites W2008574867 @default.
- W2074574257 cites W2009766076 @default.
- W2074574257 cites W2010252114 @default.
- W2074574257 cites W2012968697 @default.
- W2074574257 cites W2014715223 @default.
- W2074574257 cites W2020733002 @default.
- W2074574257 cites W2031416775 @default.
- W2074574257 cites W2034476680 @default.
- W2074574257 cites W2039849664 @default.
- W2074574257 cites W2042943718 @default.
- W2074574257 cites W2044777781 @default.
- W2074574257 cites W2047161556 @default.
- W2074574257 cites W2050376918 @default.
- W2074574257 cites W2051833845 @default.
- W2074574257 cites W2053261699 @default.
- W2074574257 cites W2053404888 @default.
- W2074574257 cites W2059394768 @default.
- W2074574257 cites W2065017189 @default.
- W2074574257 cites W2071835533 @default.
- W2074574257 cites W2086145996 @default.
- W2074574257 cites W2087935405 @default.
- W2074574257 cites W2097449851 @default.
- W2074574257 cites W2102308570 @default.
- W2074574257 cites W2104785885 @default.
- W2074574257 cites W2109902186 @default.
- W2074574257 cites W2112189758 @default.
- W2074574257 cites W2116450149 @default.
- W2074574257 cites W2132845755 @default.
- W2074574257 cites W2141413750 @default.
- W2074574257 cites W2143103411 @default.
- W2074574257 cites W2143589031 @default.
- W2074574257 cites W2146267072 @default.
- W2074574257 cites W2146893645 @default.
- W2074574257 cites W2154920883 @default.
- W2074574257 cites W2160005456 @default.
- W2074574257 cites W2162585555 @default.
- W2074574257 cites W2166996710 @default.
- W2074574257 doi "https://doi.org/10.1002/bdrc.20216" @default.
- W2074574257 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4449956" @default.
- W2074574257 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21932436" @default.
- W2074574257 hasPublicationYear "2011" @default.
- W2074574257 type Work @default.
- W2074574257 sameAs 2074574257 @default.
- W2074574257 citedByCount "28" @default.
- W2074574257 countsByYear W20745742572012 @default.
- W2074574257 countsByYear W20745742572013 @default.
- W2074574257 countsByYear W20745742572014 @default.
- W2074574257 countsByYear W20745742572015 @default.
- W2074574257 countsByYear W20745742572016 @default.
- W2074574257 countsByYear W20745742572017 @default.
- W2074574257 countsByYear W20745742572018 @default.
- W2074574257 countsByYear W20745742572019 @default.
- W2074574257 countsByYear W20745742572020 @default.
- W2074574257 countsByYear W20745742572021 @default.
- W2074574257 countsByYear W20745742572022 @default.
- W2074574257 countsByYear W20745742572023 @default.
- W2074574257 crossrefType "journal-article" @default.
- W2074574257 hasAuthorship W2074574257A5011676332 @default.
- W2074574257 hasAuthorship W2074574257A5045341517 @default.
- W2074574257 hasAuthorship W2074574257A5066935152 @default.
- W2074574257 hasAuthorship W2074574257A5079722603 @default.
- W2074574257 hasAuthorship W2074574257A5087527556 @default.
- W2074574257 hasBestOaLocation W20745742572 @default.
- W2074574257 hasConcept C102230213 @default.
- W2074574257 hasConcept C104317684 @default.
- W2074574257 hasConcept C127716648 @default.
- W2074574257 hasConcept C150194340 @default.
- W2074574257 hasConcept C161624437 @default.
- W2074574257 hasConcept C161733203 @default.
- W2074574257 hasConcept C170493617 @default.
- W2074574257 hasConcept C2776878037 @default.
- W2074574257 hasConcept C55493867 @default.
- W2074574257 hasConcept C57074206 @default.
- W2074574257 hasConcept C62478195 @default.
- W2074574257 hasConcept C74373430 @default.
- W2074574257 hasConcept C86803240 @default.
- W2074574257 hasConcept C95444343 @default.
- W2074574257 hasConceptScore W2074574257C102230213 @default.
- W2074574257 hasConceptScore W2074574257C104317684 @default.
- W2074574257 hasConceptScore W2074574257C127716648 @default.
- W2074574257 hasConceptScore W2074574257C150194340 @default.