FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2074587054> ?p ?o ?g. }

• W2074587054 endingPage "4495" @default.
• W2074587054 startingPage "4487" @default.
• W2074587054 abstract "Hepatocyte invasion by malaria parasites is mediated by specific molecular interactions. Several lines of evidence suggest the importance of the surface plasmodial circumsporozoite (CS) protein in the sporozoite invasion of hepatocytes. Identification of the sequences involved in binding to hepatocytes is an important step towards understanding the structural basis for the sporozoite-hepatocyte interaction. In this study, binding assays between Plasmodium falciparum CS peptides and HepG2 cells were performed. Fifteen overlapping residue 20 mer long peptides, spanning the entire CS sequence, were tested in HepG2 cell binding assays. Five High Binding Activity Peptides (HBAPs) to HepG2 cells were identified: 4593, (NANPNANPNANP); 4383, (NSRSLGENDDGNNEDNEKLR); 4388, (GNGQGHNMPNDPNRNVDENA); 4389, (HNMPNDPNRNVDENANANSA) and 4390, (DPNRNVDENANANSAVKNNN). The HBAP HepG2 interaction is independent of charge and amino-acid composition, but sequence dependent. Four HBAPs (4383, 4388, 4389 and 4390) are bound with similar affinity to a 50 kDa molecule. These HBAPs define three Hepatocyte Binding Sequences (HBSs): HBS-1, located between residues 68 and 87 (HBAP 4383); HBS-11, the repeat NANP region (HBAP 4593), for which anti repeat antibodies are able to specifically inhibit sporozoite invasion of hepatocytes have been reported; and HBS-111, between residues 286 and 315 (HBAPs 4388, 4388 and 4390), respectively. Interestingly, HBS 111 carries two earlier-reported B-epitopes (underlined) in peptides 4388, 4389 and 4390 (GNGQGHNMPNDPNRNVD ENANANSAVKNN) in its sequence. The HBSs reported here show lesser interspecie-variability than the entire protein in species invading the same kind of hepatic cells. This data supports these HBSs' important role in CS-protein function; they could be used as ligand by the sporozoite to invade hepatic cells." @default.
• W2074587054 created "2016-06-24" @default.
• W2074587054 creator A5007996907 @default.
• W2074587054 creator A5014298823 @default.
• W2074587054 creator A5017890836 @default.
• W2074587054 creator A5019186918 @default.
• W2074587054 creator A5019790423 @default.
• W2074587054 creator A5030090845 @default.
• W2074587054 creator A5032662640 @default.
• W2074587054 creator A5037838965 @default.
• W2074587054 creator A5042680843 @default.
• W2074587054 creator A5067379459 @default.
• W2074587054 creator A5086663478 @default.
• W2074587054 creator A5086793391 @default.
• W2074587054 date "2001-08-01" @default.
• W2074587054 modified "2023-09-26" @default.
• W2074587054 title "Plasmodium falciparum circumsporozoite (CS) protein peptides specifically bind to HepG2 cells" @default.
• W2074587054 cites W1513811097 @default.
• W2074587054 cites W1554988586 @default.
• W2074587054 cites W1734857180 @default.
• W2074587054 cites W1957867949 @default.
• W2074587054 cites W1968659851 @default.
• W2074587054 cites W1974966622 @default.
• W2074587054 cites W1984097931 @default.
• W2074587054 cites W1987377012 @default.
• W2074587054 cites W1992999474 @default.
• W2074587054 cites W1995297054 @default.
• W2074587054 cites W1995798120 @default.
• W2074587054 cites W2002568336 @default.
• W2074587054 cites W2011257176 @default.
• W2074587054 cites W2021131210 @default.
• W2074587054 cites W2021238307 @default.
• W2074587054 cites W2024718588 @default.
• W2074587054 cites W2025421232 @default.
• W2074587054 cites W2025945251 @default.
• W2074587054 cites W2027561555 @default.
• W2074587054 cites W2033090330 @default.
• W2074587054 cites W2036887760 @default.
• W2074587054 cites W2047988007 @default.
• W2074587054 cites W2051549118 @default.
• W2074587054 cites W2053948426 @default.
• W2074587054 cites W2054876551 @default.
• W2074587054 cites W2060623578 @default.
• W2074587054 cites W2065795429 @default.
• W2074587054 cites W2072592385 @default.
• W2074587054 cites W2077443612 @default.
• W2074587054 cites W2090020022 @default.
• W2074587054 cites W2091284497 @default.
• W2074587054 cites W2094954825 @default.
• W2074587054 cites W2095369948 @default.
• W2074587054 cites W2096764210 @default.
• W2074587054 cites W2097059680 @default.
• W2074587054 cites W2101498837 @default.
• W2074587054 cites W2106846584 @default.
• W2074587054 cites W2107158301 @default.
• W2074587054 cites W2112255670 @default.
• W2074587054 cites W2112336729 @default.
• W2074587054 cites W2120000058 @default.
• W2074587054 cites W2120997299 @default.
• W2074587054 cites W2124731827 @default.
• W2074587054 cites W2131460344 @default.
• W2074587054 cites W2132042285 @default.
• W2074587054 cites W2153274030 @default.
• W2074587054 cites W2155357850 @default.
• W2074587054 cites W2155731278 @default.
• W2074587054 cites W2167973356 @default.
• W2074587054 cites W2169294861 @default.
• W2074587054 cites W2320556745 @default.
• W2074587054 cites W2340524139 @default.
• W2074587054 cites W2412888032 @default.
• W2074587054 doi "https://doi.org/10.1016/s0264-410x(01)00203-1" @default.
• W2074587054 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11483275" @default.
• W2074587054 hasPublicationYear "2001" @default.
• W2074587054 type Work @default.
• W2074587054 sameAs 2074587054 @default.
• W2074587054 citedByCount "29" @default.
• W2074587054 countsByYear W20745870542012 @default.
• W2074587054 countsByYear W20745870542013 @default.
• W2074587054 countsByYear W20745870542014 @default.
• W2074587054 countsByYear W20745870542016 @default.
• W2074587054 countsByYear W20745870542017 @default.
• W2074587054 countsByYear W20745870542018 @default.
• W2074587054 countsByYear W20745870542021 @default.
• W2074587054 crossrefType "journal-article" @default.
• W2074587054 hasAuthorship W2074587054A5007996907 @default.
• W2074587054 hasAuthorship W2074587054A5014298823 @default.
• W2074587054 hasAuthorship W2074587054A5017890836 @default.
• W2074587054 hasAuthorship W2074587054A5019186918 @default.
• W2074587054 hasAuthorship W2074587054A5019790423 @default.
• W2074587054 hasAuthorship W2074587054A5030090845 @default.
• W2074587054 hasAuthorship W2074587054A5032662640 @default.
• W2074587054 hasAuthorship W2074587054A5037838965 @default.
• W2074587054 hasAuthorship W2074587054A5042680843 @default.
• W2074587054 hasAuthorship W2074587054A5067379459 @default.
• W2074587054 hasAuthorship W2074587054A5086663478 @default.
• W2074587054 hasAuthorship W2074587054A5086793391 @default.
• W2074587054 hasConcept C104317684 @default.
• W2074587054 hasConcept C107824862 @default.

• next