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• W2074628991 abstract "Pet is a cytotoxic autotransporter protein secreted by the pathogenic enteroaggregative Escherichia coli strain 042. Expression of Pet is co-dependent on two global transcription regulators: CRP (cyclic AMP receptor protein) and Fis (factor for inversion stimulation). At the pet promoter CRP binds to a single site centred at position -40.5 upstream of the start site for transcription. Due to the suboptimal positioning of this site, CRP alone activates transcription poorly and requires Fis to bind upstream to promote full activation. Here, we show that CRP and Fis control the expression of other important autotransporter toxins, namely Sat from uropathogenic E. coli (UPEC) and SigA from Shigella sonnei, and that this regulation has been conserved in different pathogens. Furthermore, we investigate the mechanism of Fis-mediated co-activation, exploiting a series of semi-synthetic promoters, with similar architecture to the pet promoter. We show that, when bound at position -40.5, CRP recruits RNA polymerase inefficiently and that Fis compensates by aiding polymerase recruitment through a direct protein–protein interaction. We demonstrate that other suitably positioned upstream transcription factors, which directly recruit RNA polymerase, can also compensate for the inappropriate positioning of CRP. We propose that this is a simple ‘shared-recruitment’ mechanism, by which co-dependence of promoters on two transcription factors could evolve." @default.
• W2074628991 created "2016-06-24" @default.
• W2074628991 creator A5030866254 @default.
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• W2074628991 creator A5047017617 @default.
• W2074628991 creator A5056094308 @default.
• W2074628991 date "2015-02-20" @default.
• W2074628991 modified "2023-09-27" @default.
• W2074628991 title "Expression of different bacterial cytotoxins is controlled by two global transcription factors, CRP and Fis, that co-operate in a shared-recruitment mechanism" @default.
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• W2074628991 doi "https://doi.org/10.1042/bj20141315" @default.
• W2074628991 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25484033" @default.
• W2074628991 hasPublicationYear "2015" @default.
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