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• W2074654002 abstract "The cylindrical Hsp100 chaperone ClpA mediates ATP-dependent unfolding of substrate proteins bearing tag sequences, such as the 11-residue ssrA sequence appended to proteins translationally stalled at ribosomes. Unfolding is coupled to translocation through a central channel into the associating protease, ClpP. To explore the topology and mechanism of ClpA action, we carried out chemical crosslinking and functional studies. Whereas a tag from RepA protein crosslinked proximally to the flexible N domains, the ssrA sequence in GFP-ssrA crosslinked distally in the channel to a segment of the distal ATPase domain (D2). Single substitutions placed in this D2 loop, and also in two apparently cooperating proximal (D1) loops, abolished binding of ssrA substrates and unfolded proteins lacking tags and blocked unfolding of GFP-RepA. Additionally, a substitution adjoining the D2 loop allowed binding of ssrA proteins but impaired their translocation. This loop, as in homologous nucleic-acid translocases, may bind substrates proximally and, coupled with ATP hydrolysis, translocate them distally, exerting mechanical force that mediates unfolding." @default.
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• W2074654002 date "2005-07-01" @default.
• W2074654002 modified "2023-10-16" @default.
• W2074654002 title "Loops in the Central Channel of ClpA Chaperone Mediate Protein Binding, Unfolding, and Translocation" @default.
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• W2074654002 doi "https://doi.org/10.1016/j.cell.2005.04.012" @default.
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