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• W2074654540 endingPage "725" @default.
• W2074654540 startingPage "717" @default.
• W2074654540 abstract "Acholeplasma laidlawii cells were grown in cholesterol-enriched medium and exposed continuously to either air (control), 4.0 vol.% halothane in air at 1 atm pressure (4% atm halothane), or 80% cyclopropane in oxygen for 24 h at 37 degrees C. Cells grown in the presence of 4% atm halothane or 80% cyclopropane had approximately twice as much membrane cholesterol content/mg protein as the control cells. Cells grown in an anesthetic environment also tended to have a higher membrane cholesterol/phospholipid molar ratio compared to control cells. Membranes isolated from halothane-exposed cells grown in a cholesterol-enriched medium were more ordered at 37 degrees C (measurements were made with no anesthetic present) than membranes from control cells grown in an identically enriched medium. This difference in membrane physical state between control and anesthetic-exposed cells decreased as the temperature decreased, and disappeared at approx. 23 degrees C. Continuous exposure of A. laidlawii to 4% atm halothane or 80% cyclopropane for 24 h did not markedly affect membrane fatty acid composition, either in cells grown on an unsupplemented medium or in cells grown in a medium enriched in myristic, palmitic or stearic acids. These results further support the hypothesis that an increased membrane cholesterol content may play a role in the tolerance or dependence that develops after chronic exposure to anesthetic agents." @default.
• W2074654540 created "2016-06-24" @default.
• W2074654540 creator A5047166116 @default.
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• W2074654540 date "1981-12-01" @default.
• W2074654540 modified "2023-09-24" @default.
• W2074654540 title "Chronic exposure to inhaled anesthetics increases cholesterol content in Acholeplasma laidlawii" @default.
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• W2074654540 doi "https://doi.org/10.1016/0005-2736(81)90176-0" @default.
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