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• W2074665891 abstract "To understand the DNA binding mechanism ofAtSPL14 protein, the imino proton exchange rates weremeasured for the DNA duplex containing the consensusDNA-binding site for the AtSPL14 transcription factor(referred to as wt-SPL14 duplex, Fig. 1). To further under-stand the correlation between the base pair stability/dynamicsand DNA binding affinity of the AtSPL14, the exchange rateconstants of the imino protons for the wt-SPL14 duplexwere compared with those of the mutant SPL14 duplexes(see Fig. 1), which display different binding affinities forAtSPL14 protein. Experimental SectionAll DNA oligonucleotides were purchased from M-bio-tech Co. (Seoul, Korea). The oligonucleotides were purifiedby reverse-phase HPLC and desalted by Sephadex G-25 column.DNA duplexes were prepared by dissolving two strands at a1:1 stoichiometric ratio in an NMR buffer (90% H" @default.
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• W2074665891 date "2013-08-20" @default.
• W2074665891 modified "2023-09-24" @default.
• W2074665891 title "Hydrogen Exchange Study of DNA Duplexes Containing the Consensus Binding Site for Arabidopsis thaliana SPL14 Transcription Factor" @default.
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• W2074665891 doi "https://doi.org/10.5012/bkcs.2013.34.8.2511" @default.
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