Matches in SemOpenAlex for { <https://semopenalex.org/work/W2074668545> ?p ?o ?g. }
- W2074668545 endingPage "450.e1" @default.
- W2074668545 startingPage "441" @default.
- W2074668545 abstract "Background Treatment with low-molecular-weight heparin (LMWH) favorably alters the vein wall response to deep venous thrombosis (DVT), although the mechanisms remain unclear. Previous studies have suggested that LMWH alters the levels of circulating plasminogen activator inhibitor 1 (PAI-1), a known mediator of fibrosis, and may improve endogenous fibrinolysis. We hypothesized that LMWH favorably alters the vein wall response by binding of PAI-1 and acceleration of fibrinolysis. Methods Wild-type and PAI-1−/− mice underwent treatment with LMWH after induction of occlusive DVT. Vein wall and plasma were harvested and analyzed by enzyme-linked immunosorbent assay, zymography, real-time polymerase chain reaction, and immunohistochemistry. Results Wild-type mice treated with LMWH exhibited diminished vein wall fibrosis (0.6 ± 0.6 vs 1.4 ± 0.2; P < .01; n = 5) and elevation of circulating PAI-1 (1776 ± 342 vs 567 ± 104 ρg/mL; P < .01; n = 5) compared with untreated controls after occlusive DVT. PAI-1−/− mice treated with LMWH were not similarly protected from fibrosis, despite improved thrombus resolution. Treatment with LMWH was associated with decreased intrathrombus interleukin-1β (68.6 ± 31.0 vs 223.4 ± 28.9 ρg/mg total protein; P < .01; n = 5) but did not alter inflammatory cell recruitment to the vein wall. PAI-1−/− mice exhibited significantly elevated intrathrombus (257.2 ± 51.5 vs 14.3 ± 3.8 ρg/mg total protein; n = 5) and vein wall interleukin-13 (187.2 ± 57.6 vs 9.9 ± 1.1 ρg/mg total protein; P < .05; n = 5) as well as vein wall F4/80 positively staining monocytes (53 ± 11 vs 16 ± 2 cells/5 high-power fields; P < .05; n = 4). Conclusions LMWH did not accelerate venous thrombosis resolution but did protect against vein wall fibrosis in a PAI-1-dependent manner in an occlusive DVT model. Lack of PAI-1 correlated with accelerated venous thrombosis resolution but no protection from fibrosis. PAI-1 inhibition as a treatment strategy for DVT is likely to accelerate clearance of the thrombus but may come at the expense of increased vein wall fibrosis. Treatment with low-molecular-weight heparin (LMWH) favorably alters the vein wall response to deep venous thrombosis (DVT), although the mechanisms remain unclear. Previous studies have suggested that LMWH alters the levels of circulating plasminogen activator inhibitor 1 (PAI-1), a known mediator of fibrosis, and may improve endogenous fibrinolysis. We hypothesized that LMWH favorably alters the vein wall response by binding of PAI-1 and acceleration of fibrinolysis. Wild-type and PAI-1−/− mice underwent treatment with LMWH after induction of occlusive DVT. Vein wall and plasma were harvested and analyzed by enzyme-linked immunosorbent assay, zymography, real-time polymerase chain reaction, and immunohistochemistry. Wild-type mice treated with LMWH exhibited diminished vein wall fibrosis (0.6 ± 0.6 vs 1.4 ± 0.2; P < .01; n = 5) and elevation of circulating PAI-1 (1776 ± 342 vs 567 ± 104 ρg/mL; P < .01; n = 5) compared with untreated controls after occlusive DVT. PAI-1−/− mice treated with LMWH were not similarly protected from fibrosis, despite improved thrombus resolution. Treatment with LMWH was associated with decreased intrathrombus interleukin-1β (68.6 ± 31.0 vs 223.4 ± 28.9 ρg/mg total protein; P < .01; n = 5) but did not alter inflammatory cell recruitment to the vein wall. PAI-1−/− mice exhibited significantly elevated intrathrombus (257.2 ± 51.5 vs 14.3 ± 3.8 ρg/mg total protein; n = 5) and vein wall interleukin-13 (187.2 ± 57.6 vs 9.9 ± 1.1 ρg/mg total protein; P < .05; n = 5) as well as vein wall F4/80 positively staining monocytes (53 ± 11 vs 16 ± 2 cells/5 high-power fields; P < .05; n = 4). LMWH did not accelerate venous thrombosis resolution but did protect against vein wall fibrosis in a PAI-1-dependent manner in an occlusive DVT model. Lack of PAI-1 correlated with accelerated venous thrombosis resolution but no protection from fibrosis. PAI-1 inhibition as a treatment strategy for DVT is likely to accelerate clearance of the thrombus but may come at the expense of increased vein wall fibrosis." @default.
- W2074668545 created "2016-06-24" @default.
- W2074668545 creator A5010930677 @default.
- W2074668545 creator A5026298390 @default.
- W2074668545 creator A5056894258 @default.
- W2074668545 creator A5058935102 @default.
- W2074668545 creator A5063870060 @default.
- W2074668545 creator A5075997575 @default.
- W2074668545 creator A5078752894 @default.
- W2074668545 creator A5090323418 @default.
- W2074668545 date "2014-10-01" @default.
- W2074668545 modified "2023-09-29" @default.
- W2074668545 title "Low-molecular-weight heparin modulates vein wall fibrotic response in a plasminogen activator inhibitor 1-dependent manner" @default.
- W2074668545 cites W1551336557 @default.
- W2074668545 cites W1900486712 @default.
- W2074668545 cites W1974655370 @default.
- W2074668545 cites W1974725320 @default.
- W2074668545 cites W1978764415 @default.
- W2074668545 cites W1979664199 @default.
- W2074668545 cites W1981287243 @default.
- W2074668545 cites W1984803858 @default.
- W2074668545 cites W1985553563 @default.
- W2074668545 cites W1985770941 @default.
- W2074668545 cites W2002354857 @default.
- W2074668545 cites W2005388913 @default.
- W2074668545 cites W2014956686 @default.
- W2074668545 cites W2024441465 @default.
- W2074668545 cites W2025845709 @default.
- W2074668545 cites W2029383045 @default.
- W2074668545 cites W2043976304 @default.
- W2074668545 cites W2049404164 @default.
- W2074668545 cites W2064849355 @default.
- W2074668545 cites W2071395909 @default.
- W2074668545 cites W2072477938 @default.
- W2074668545 cites W2076291626 @default.
- W2074668545 cites W2081415617 @default.
- W2074668545 cites W2090074864 @default.
- W2074668545 cites W2092519919 @default.
- W2074668545 cites W2114788433 @default.
- W2074668545 cites W2114885288 @default.
- W2074668545 cites W2123599115 @default.
- W2074668545 cites W2131099415 @default.
- W2074668545 cites W2141672986 @default.
- W2074668545 cites W2142102042 @default.
- W2074668545 cites W2154952909 @default.
- W2074668545 cites W2155364931 @default.
- W2074668545 cites W2156644795 @default.
- W2074668545 cites W2156816596 @default.
- W2074668545 cites W2157553988 @default.
- W2074668545 cites W2169347963 @default.
- W2074668545 cites W2169673832 @default.
- W2074668545 cites W4247171926 @default.
- W2074668545 cites W4285493257 @default.
- W2074668545 cites W4295715489 @default.
- W2074668545 cites W2037028641 @default.
- W2074668545 doi "https://doi.org/10.1016/j.jvsv.2014.02.004" @default.
- W2074668545 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4235166" @default.
- W2074668545 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25419511" @default.
- W2074668545 hasPublicationYear "2014" @default.
- W2074668545 type Work @default.
- W2074668545 sameAs 2074668545 @default.
- W2074668545 citedByCount "13" @default.
- W2074668545 countsByYear W20746685452014 @default.
- W2074668545 countsByYear W20746685452016 @default.
- W2074668545 countsByYear W20746685452017 @default.
- W2074668545 countsByYear W20746685452020 @default.
- W2074668545 countsByYear W20746685452022 @default.
- W2074668545 crossrefType "journal-article" @default.
- W2074668545 hasAuthorship W2074668545A5010930677 @default.
- W2074668545 hasAuthorship W2074668545A5026298390 @default.
- W2074668545 hasAuthorship W2074668545A5056894258 @default.
- W2074668545 hasAuthorship W2074668545A5058935102 @default.
- W2074668545 hasAuthorship W2074668545A5063870060 @default.
- W2074668545 hasAuthorship W2074668545A5075997575 @default.
- W2074668545 hasAuthorship W2074668545A5078752894 @default.
- W2074668545 hasAuthorship W2074668545A5090323418 @default.
- W2074668545 hasBestOaLocation W20746685452 @default.
- W2074668545 hasConcept C109523444 @default.
- W2074668545 hasConcept C126322002 @default.
- W2074668545 hasConcept C134018914 @default.
- W2074668545 hasConcept C177430391 @default.
- W2074668545 hasConcept C2776825266 @default.
- W2074668545 hasConcept C2776884760 @default.
- W2074668545 hasConcept C2777557582 @default.
- W2074668545 hasConcept C2779679481 @default.
- W2074668545 hasConcept C2780559512 @default.
- W2074668545 hasConcept C2780675426 @default.
- W2074668545 hasConcept C2781287897 @default.
- W2074668545 hasConcept C2781362458 @default.
- W2074668545 hasConcept C71924100 @default.
- W2074668545 hasConceptScore W2074668545C109523444 @default.
- W2074668545 hasConceptScore W2074668545C126322002 @default.
- W2074668545 hasConceptScore W2074668545C134018914 @default.
- W2074668545 hasConceptScore W2074668545C177430391 @default.
- W2074668545 hasConceptScore W2074668545C2776825266 @default.
- W2074668545 hasConceptScore W2074668545C2776884760 @default.
- W2074668545 hasConceptScore W2074668545C2777557582 @default.
- W2074668545 hasConceptScore W2074668545C2779679481 @default.