Matches in SemOpenAlex for { <https://semopenalex.org/work/W2074807743> ?p ?o ?g. }
Showing items 1 to 54 of
54
with 100 items per page.
- W2074807743 endingPage "12" @default.
- W2074807743 startingPage "1" @default.
- W2074807743 abstract "Rapid fluorimetric methods are described which involve six enzyme systems for the determination of mixtures of 21 organic acids. Lactate (Types II and IV), malate, glutamate, isocitrate, and β-hydroxybutyrate dehydrogenases are used, coupled with NAD, phenazine methosulfate and resazurin, in a fluorimetric procedure for the determination of acetic, adipic, benzilic, butyric, D-α - and D-β-hydroxybutyric, chloroacetic, DL-citric, formic, L-glutamic, glutaric, glycolic, threo-D-isocitric, L-lactic, L-malic, malonic, oxalic, phthalic, DL-succinic and L-tartaric acids in the approximate concentration range of 0.1–500 μg with an accuracy and precision of about 2%. The rate of production of the highly fluorescent resorufin is equated to the concentration of the acid. On décrit des méthodes fluorimétriques avec enzymes pour le dosage de mélanges d'acides organiques. On utilise des déhydrogénases de lactate (types II et IV), malate, glutamate, isocitrate et β-hydroxybutyrate, avec NAD, phénazine métosulfate et résazurine, pour le dosage des acides acétique, adipique, benzylique, butyrique, D-α- et D-β -hydroxybutyrique, chloroacétique, DL-citrique, formique, L-glutamique, glutarique, glycolique, threo-D-isocitrique, L-lactique, L-malique, malonique, oxalique, phtalique, DL-succinique et L-tartrique, à une concentration de 0.1 à 500 μg avec une exactitude et une précision d'environ 2%. La vitesse de production de la résorufine (fluorescence) est fonction de la concentration de l'acide. Es wird eine schnelle fluorimctrische Methode beschrieben, die enzymatische Systeme zur Bestimmung von Mischungen von 21 organischen Säuren verwendet. Lactat, Malat, Glutamat, Isocitrat und β-Hydroxybutyrat-Dehydrogenase werden verwendet zusammen mit NAD, Phenazinmethosulfat und Resazurin in einem fluorimetrischen Verfahren zur Bestimmung der verschiedensten Säuren. Im Kon-zentrationsbereich von etwa 0.1–500 μg betragen die Reproduzierbarkeit und Richtigkeit etwa 2%. Die Bildungsrate des sehr stark fluoreszierenden Resorufins ist etwa gleich der Konzentration der Säure." @default.
- W2074807743 created "2016-06-24" @default.
- W2074807743 creator A5000961244 @default.
- W2074807743 creator A5007323800 @default.
- W2074807743 creator A5022354452 @default.
- W2074807743 date "1969-03-01" @default.
- W2074807743 modified "2023-10-16" @default.
- W2074807743 title "A fluorimetric enzymic method for the assay of mixtures of organic acids" @default.
- W2074807743 cites W1998651823 @default.
- W2074807743 cites W2021373297 @default.
- W2074807743 cites W2404294769 @default.
- W2074807743 cites W4238932965 @default.
- W2074807743 cites W4246239289 @default.
- W2074807743 doi "https://doi.org/10.1016/s0003-2670(00)89380-0" @default.
- W2074807743 hasPublicationYear "1969" @default.
- W2074807743 type Work @default.
- W2074807743 sameAs 2074807743 @default.
- W2074807743 citedByCount "20" @default.
- W2074807743 countsByYear W20748077432014 @default.
- W2074807743 crossrefType "journal-article" @default.
- W2074807743 hasAuthorship W2074807743A5000961244 @default.
- W2074807743 hasAuthorship W2074807743A5007323800 @default.
- W2074807743 hasAuthorship W2074807743A5022354452 @default.
- W2074807743 hasConcept C178790620 @default.
- W2074807743 hasConcept C181199279 @default.
- W2074807743 hasConcept C185592680 @default.
- W2074807743 hasConcept C43617362 @default.
- W2074807743 hasConcept C75520062 @default.
- W2074807743 hasConceptScore W2074807743C178790620 @default.
- W2074807743 hasConceptScore W2074807743C181199279 @default.
- W2074807743 hasConceptScore W2074807743C185592680 @default.
- W2074807743 hasConceptScore W2074807743C43617362 @default.
- W2074807743 hasConceptScore W2074807743C75520062 @default.
- W2074807743 hasIssue "1" @default.
- W2074807743 hasLocation W20748077431 @default.
- W2074807743 hasOpenAccess W2074807743 @default.
- W2074807743 hasPrimaryLocation W20748077431 @default.
- W2074807743 hasRelatedWork W1546333328 @default.
- W2074807743 hasRelatedWork W1974801319 @default.
- W2074807743 hasRelatedWork W2089528991 @default.
- W2074807743 hasRelatedWork W2093376715 @default.
- W2074807743 hasRelatedWork W2174136156 @default.
- W2074807743 hasRelatedWork W2277076226 @default.
- W2074807743 hasRelatedWork W2903308772 @default.
- W2074807743 hasRelatedWork W3022082568 @default.
- W2074807743 hasRelatedWork W3118323830 @default.
- W2074807743 hasRelatedWork W4292367286 @default.
- W2074807743 hasVolume "45" @default.
- W2074807743 isParatext "false" @default.
- W2074807743 isRetracted "false" @default.
- W2074807743 magId "2074807743" @default.
- W2074807743 workType "article" @default.