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- W2074865301 abstract "MPB70 and MPB83 are among the most characteristically exported proteins defining a strongly expressed phenotype of Mycobacterium bovis . These proteins are known to be homologous to osteoblast‐specific factor 2. By in vitro culture of mycobacteria they appear to have a limited species distribution and to be relatively specific for M. bovis. Virtually identical genes are however, present in Mycobacterium tuberculosis . In order to facilitate further research into the immunobiology of these proteins and their potential application for differential diagnosis of tuberculosis as a result of M. bovis , we describe the reactivities of 20 monoclonal antibodies (MoAbs) to these proteins. Immunizing with bovine PPD generated 10 MoAbs. These antibodies reacted preferentially with the soluble MPB70 antigen using reducing conditions in SDS‐PAGE with western blotting. Ten MoAbs were generated by immunizing mice with fractions derived from a whole cell sonic extract of M. bovis . These antibodies reacted preferentially with the surface exposed MPB83 lipoglycoprotein." @default.
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- W2074865301 date "2001-05-01" @default.
- W2074865301 modified "2023-10-18" @default.
- W2074865301 title "Novel Monoclonal Antibodies against Major Antigens of <i>Mycobacterium bovis</i>" @default.
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- W2074865301 doi "https://doi.org/10.1046/j.1365-3083.2001.00914.x" @default.
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