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- W2074865624 abstract "During mitosis in budding yeast, cortically anchored dynein generates pulling forces on astral microtubules to position the mitotic spindle across the mother–bud neck. The attachment molecule Num1 is required for dynein anchoring at the cell membrane, but how Num1 assembles into stationary cortical patches and interacts with dynein is unknown. We show that an N-terminal Bin/Amphiphysin/Rvs (BAR)–like domain in Num1 mediates the assembly of morphologically distinct patches and its interaction with dynein for spindle translocation into the bud. We name this domain patch assembly domain (PA; residues 1–303), as it was both necessary and sufficient for the formation of functional dynein-anchoring patches when it was attached to a pleckstrin homology domain or a CAAX motif. Distinct point mutations targeting the predicted BAR-like PA domain differentially disrupted patch assembly, dynein anchoring, and mitochondrial attachment functions of Num1. We also show that the PA domain is an elongated dimer and discuss the mechanism by which it drives patch assembly." @default.
- W2074865624 created "2016-06-24" @default.
- W2074865624 creator A5019137664 @default.
- W2074865624 creator A5034085444 @default.
- W2074865624 creator A5091213639 @default.
- W2074865624 date "2012-03-19" @default.
- W2074865624 modified "2023-10-18" @default.
- W2074865624 title "A novel patch assembly domain in Num1 mediates dynein anchoring at the cortex during spindle positioning" @default.
- W2074865624 cites W1533372454 @default.
- W2074865624 cites W1561680043 @default.
- W2074865624 cites W1570169904 @default.
- W2074865624 cites W1667488495 @default.
- W2074865624 cites W1963759286 @default.
- W2074865624 cites W1965475092 @default.
- W2074865624 cites W1966072209 @default.
- W2074865624 cites W1973275541 @default.
- W2074865624 cites W1973756205 @default.
- W2074865624 cites W1976964790 @default.
- W2074865624 cites W1977287344 @default.
- W2074865624 cites W1981559490 @default.
- W2074865624 cites W1983036595 @default.
- W2074865624 cites W1990341894 @default.
- W2074865624 cites W1993893387 @default.
- W2074865624 cites W1996888932 @default.
- W2074865624 cites W1999286862 @default.
- W2074865624 cites W1999768397 @default.
- W2074865624 cites W2004344365 @default.
- W2074865624 cites W2008003875 @default.
- W2074865624 cites W2015224745 @default.
- W2074865624 cites W2018745023 @default.
- W2074865624 cites W2019593540 @default.
- W2074865624 cites W2022391435 @default.
- W2074865624 cites W2029907860 @default.
- W2074865624 cites W2033361228 @default.
- W2074865624 cites W2036692489 @default.
- W2074865624 cites W2037312364 @default.
- W2074865624 cites W2038970717 @default.
- W2074865624 cites W2044668046 @default.
- W2074865624 cites W2044713093 @default.
- W2074865624 cites W2045843159 @default.
- W2074865624 cites W2046561940 @default.
- W2074865624 cites W2050688574 @default.
- W2074865624 cites W2052166374 @default.
- W2074865624 cites W2052740445 @default.
- W2074865624 cites W2053527054 @default.
- W2074865624 cites W2054836163 @default.
- W2074865624 cites W2066353180 @default.
- W2074865624 cites W2071877471 @default.
- W2074865624 cites W2073921638 @default.
- W2074865624 cites W2076730474 @default.
- W2074865624 cites W2078146767 @default.
- W2074865624 cites W2083704406 @default.
- W2074865624 cites W2094435901 @default.
- W2074865624 cites W2096718270 @default.
- W2074865624 cites W2096918142 @default.
- W2074865624 cites W2102294470 @default.
- W2074865624 cites W2104180471 @default.
- W2074865624 cites W2108586733 @default.
- W2074865624 cites W2116846184 @default.
- W2074865624 cites W2119114654 @default.
- W2074865624 cites W2122401596 @default.
- W2074865624 cites W2125089139 @default.
- W2074865624 cites W2126667110 @default.
- W2074865624 cites W2133664013 @default.
- W2074865624 cites W2135607368 @default.
- W2074865624 cites W2137089508 @default.
- W2074865624 cites W2138882854 @default.
- W2074865624 cites W2140896223 @default.
- W2074865624 cites W2143456993 @default.
- W2074865624 cites W2144996654 @default.
- W2074865624 cites W2146191462 @default.
- W2074865624 cites W2146750095 @default.
- W2074865624 cites W2146831574 @default.
- W2074865624 cites W2149151220 @default.
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- W2074865624 cites W2158799849 @default.
- W2074865624 cites W2159460045 @default.
- W2074865624 cites W2164372658 @default.
- W2074865624 cites W2164702837 @default.
- W2074865624 cites W2165843409 @default.
- W2074865624 cites W2167142138 @default.
- W2074865624 cites W2170535602 @default.
- W2074865624 cites W2171909588 @default.
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- W2074865624 doi "https://doi.org/10.1083/jcb.201112017" @default.
- W2074865624 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3308694" @default.
- W2074865624 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22431751" @default.
- W2074865624 hasPublicationYear "2012" @default.
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