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- W2074889669 abstract "For many years urinary hydroxyproline (OHPr) has been used to assess collagen metabolism, collagen being the only protein of the human body containing this imino acid. Most methods are modifications of the principle first described by Lang in 1933: oxidation of OHPr to pyrrole followed by a coupling with p-dimethylaminobenzaldehyde [l]. Moreover, many of the published methods are inaccurate and made complicated by non-specific agents which produce interfering chromophores [2-61. This has necessitated subtraction of blank values which have been reported to constitute up to 74% of the total colour intensity [7]. In our department we have used analyses of urinary OHPr as part of an evaluation of bone metabolism in many subjects. The many samples stimulated interest in improving the analysis. We introduce here a new method, which in a simple way by an ‘acid-base’ clearing procedure effectively removes interfering chromophores." @default.
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- W2074889669 date "1984-09-01" @default.
- W2074889669 modified "2023-10-17" @default.
- W2074889669 title "An easy and reliable method for determination of urinary hydroxyproline" @default.
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- W2074889669 doi "https://doi.org/10.1016/0009-8981(84)90110-4" @default.
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