FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2074995350> ?p ?o ?g. }

• W2074995350 endingPage "23" @default.
• W2074995350 startingPage "13" @default.
• W2074995350 abstract "Interactions of heparin with intact human thrombospondin-1 (TSP1) and with two heparin-binding fragments of TSP1 were characterized using chemically modified heparins, a vascular heparan sulfate proteoglycan, and a series of heparin oligosaccharides prepared by partial deaminative cleavage. The avidity of TSP1 binding increased with oligosaccharide size, with plateaus at 4 to 6 and at 8 to 10 monosaccharide units. The dependence on oligosaccharide size for binding to the recombinant amino-terminal heparin-binding domain of TSP1 was the same as that of the intact TSP1 molecule but differed from that of a synthetic heparin-binding peptide from the type 1 repeats, suggesting that the interaction between intact TSP1 and heparin is primarily mediated by the amino-terminal domain. Based on activities of chemically modified heparins, binding to TSP1 depended primarily on 2-N- and 6-O-sulfation of glucosamine and to a lesser degree on 2,3-O-sulfation and the carboxyl residues of the uronic acids. In contrast, all of these modifications were required for binding of heparin to the type 1 repeat peptides. Affinity purification of heparin octasaccharides on immobilized TSP1 type 1 repeat peptides revealed a preference for oligosaccharides containing the disaccharide sequence IdoA(2-OSO(3))alpha1-4-GlcNS(6-OSO(3)). Binding of these oligosaccharides to the peptide required the Trp residues. These data demonstrate that the heparin-binding specificities of intact TSP1 and peptides from the type 1 repeats overlap with that of basic fibroblast growth factor (FGF2) and are consistent with the ability of these TSP1-derived molecules to inhibit FGF2-stimulated angiogenesis." @default.
• W2074995350 created "2016-06-24" @default.
• W2074995350 creator A5009736406 @default.
• W2074995350 creator A5010648900 @default.
• W2074995350 creator A5025235827 @default.
• W2074995350 creator A5030642152 @default.
• W2074995350 creator A5031078822 @default.
• W2074995350 creator A5034054391 @default.
• W2074995350 creator A5043047301 @default.
• W2074995350 creator A5043504060 @default.
• W2074995350 creator A5049513991 @default.
• W2074995350 creator A5052759817 @default.
• W2074995350 creator A5067555795 @default.
• W2074995350 creator A5070044197 @default.
• W2074995350 date "2000-02-01" @default.
• W2074995350 modified "2023-09-26" @default.
• W2074995350 title "Specificities of Heparin-binding Sites from the Amino-Terminus and Type 1 Repeats of Thrombospondin-1" @default.
• W2074995350 cites W1486180443 @default.
• W2074995350 cites W1486368456 @default.
• W2074995350 cites W1490866125 @default.
• W2074995350 cites W1494551606 @default.
• W2074995350 cites W1523736322 @default.
• W2074995350 cites W1534240923 @default.
• W2074995350 cites W1540432147 @default.
• W2074995350 cites W1541903924 @default.
• W2074995350 cites W1559464451 @default.
• W2074995350 cites W1568527001 @default.
• W2074995350 cites W1578995377 @default.
• W2074995350 cites W1595861603 @default.
• W2074995350 cites W1597493507 @default.
• W2074995350 cites W1598116581 @default.
• W2074995350 cites W1603286011 @default.
• W2074995350 cites W1636802518 @default.
• W2074995350 cites W1877090369 @default.
• W2074995350 cites W1934065093 @default.
• W2074995350 cites W1941829554 @default.
• W2074995350 cites W1964680859 @default.
• W2074995350 cites W1965909442 @default.
• W2074995350 cites W1967043982 @default.
• W2074995350 cites W1970022187 @default.
• W2074995350 cites W1972078597 @default.
• W2074995350 cites W1972621645 @default.
• W2074995350 cites W1979566489 @default.
• W2074995350 cites W1982952302 @default.
• W2074995350 cites W1983956381 @default.
• W2074995350 cites W1985009903 @default.
• W2074995350 cites W1998596090 @default.
• W2074995350 cites W2007799501 @default.
• W2074995350 cites W2007970283 @default.
• W2074995350 cites W2014771072 @default.
• W2074995350 cites W2016391435 @default.
• W2074995350 cites W2020011204 @default.
• W2074995350 cites W2020206304 @default.
• W2074995350 cites W2022665089 @default.
• W2074995350 cites W2029305252 @default.
• W2074995350 cites W2031354590 @default.
• W2074995350 cites W2040449827 @default.
• W2074995350 cites W2041420763 @default.
• W2074995350 cites W2043813981 @default.
• W2074995350 cites W2047157544 @default.
• W2074995350 cites W2056105561 @default.
• W2074995350 cites W2059322719 @default.
• W2074995350 cites W2064239932 @default.
• W2074995350 cites W2066161150 @default.
• W2074995350 cites W2066936269 @default.
• W2074995350 cites W2068473054 @default.
• W2074995350 cites W2096415735 @default.
• W2074995350 cites W2096880046 @default.
• W2074995350 cites W2100468028 @default.
• W2074995350 cites W2101650166 @default.
• W2074995350 cites W2132082955 @default.
• W2074995350 cites W2136845216 @default.
• W2074995350 cites W2149047900 @default.
• W2074995350 cites W2154086195 @default.
• W2074995350 cites W2162927078 @default.
• W2074995350 cites W2165233782 @default.
• W2074995350 cites W2167899389 @default.
• W2074995350 cites W2233811338 @default.
• W2074995350 doi "https://doi.org/10.1006/abbi.1999.1597" @default.
• W2074995350 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10640391" @default.
• W2074995350 hasPublicationYear "2000" @default.
• W2074995350 type Work @default.
• W2074995350 sameAs 2074995350 @default.
• W2074995350 citedByCount "28" @default.
• W2074995350 countsByYear W20749953502014 @default.
• W2074995350 countsByYear W20749953502015 @default.
• W2074995350 countsByYear W20749953502016 @default.
• W2074995350 countsByYear W20749953502017 @default.
• W2074995350 countsByYear W20749953502020 @default.
• W2074995350 countsByYear W20749953502021 @default.
• W2074995350 countsByYear W20749953502022 @default.
• W2074995350 crossrefType "journal-article" @default.
• W2074995350 hasAuthorship W2074995350A5009736406 @default.
• W2074995350 hasAuthorship W2074995350A5010648900 @default.
• W2074995350 hasAuthorship W2074995350A5025235827 @default.
• W2074995350 hasAuthorship W2074995350A5030642152 @default.
• W2074995350 hasAuthorship W2074995350A5031078822 @default.
• W2074995350 hasAuthorship W2074995350A5034054391 @default.

• next