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- W2075015685 abstract "Recently, Vakonakis and LiWang (J. Am. Chem. Soc. 2004, 126, 5688) reported experimental evidence for stronger hydrogen bonds in RNA A:U than in DNA A:T base pairs, which was based on differences in NMR shielding for adenine C2. We have analyzed the proposed correlation between NMR shielding and hydrogen-bond strength using density functional theory. Although we agree with the conclusion that A:U is more strongly bound, we find no correlation between the hydrogen-bond strength and the NMR shielding of C2. Our study shows that NMR merely probes the presence/absence of the methyl group in thymine/uracil, without any relation to the strength of the hydrogen bonds involved. In other words, one cannot infer the Watson−Crick hydrogen-bond strength from the NMR shielding constant of adenine C2." @default.
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- W2075015685 date "2004-12-01" @default.
- W2075015685 modified "2023-10-12" @default.
- W2075015685 title "Hydrogen Bonds of RNA Are Stronger than Those of DNA, but NMR Monitors Only Presence of Methyl Substituent in Uracil/Thymine" @default.
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- W2075015685 doi "https://doi.org/10.1021/ja045276b" @default.
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