Matches in SemOpenAlex for { <https://semopenalex.org/work/W2075046091> ?p ?o ?g. }
- W2075046091 endingPage "10" @default.
- W2075046091 startingPage "1" @default.
- W2075046091 abstract "The speciation of dissolved copper in surface seawater is dominated by complexes with at least two classes of organic ligands, L1 (the stronger) and L2 (the weaker). However, because these ligands have never been isolated, recovered, and characterized, their nature and identities are unknown. We have taken a combined approach utilizing immobilized metal affinity chromatography (IMAC) for isolation, and copper titration monitored by differential pulse anodic stripping voltammetry (DPASV) at a thin mercury film, rotating glassy carbon disk electrode for detection of these ligands in marine waters and in chromatographic fractions. We have found that, using the IMAC protocol reported by A.S. Gordon (Isolation of compounds with affinity for copper from seawater using immobilized copper ion affinity chromatography, Mar. Chem. 38 (1992) 1–12), we can remove the majority of L1 and L2 from Chesapeake Bay and Atlantic coastal water samples. Once isolated, ~ 67% of the L1-class ligands bound to the IMAC column could be recovered by elution with 5 m M glycine buffer, as monitored by DPASV copper titration of the column eluent. This recovery accounted for ~ 47% of the original amount of L1 in the sample and resulted in a 5.4-fold concentration factor (from 7 to 38 n M) of the ligands from the seawater sample into glycine eluent. Although this IMAC protocol traps the weaker, L2 class ligands as well as L1 class ligands, confirmation of L2's recovery by elution of the IMAC column is hindered by the presence of glycine in the eluent. We are currently devising procedures to optimize the isolation and improve recovery efficiency of IMAC for these ligands, in order to obtain sufficient quantities for further characterization." @default.
- W2075046091 created "2016-06-24" @default.
- W2075046091 creator A5016341426 @default.
- W2075046091 creator A5052033048 @default.
- W2075046091 creator A5068824664 @default.
- W2075046091 date "1997-05-01" @default.
- W2075046091 modified "2023-10-16" @default.
- W2075046091 title "Evaluation of immobilized metal affinity chromatography (IMAC) for isolation and recovery of strong copper-complexing ligands from marine waters" @default.
- W2075046091 cites W1974796647 @default.
- W2075046091 cites W1990101686 @default.
- W2075046091 cites W1998617755 @default.
- W2075046091 cites W2001635884 @default.
- W2075046091 cites W2001861274 @default.
- W2075046091 cites W2003470884 @default.
- W2075046091 cites W2018158276 @default.
- W2075046091 cites W2028446963 @default.
- W2075046091 cites W2031702546 @default.
- W2075046091 cites W2038269836 @default.
- W2075046091 cites W2038778927 @default.
- W2075046091 cites W2040962971 @default.
- W2075046091 cites W2041261873 @default.
- W2075046091 cites W2048510103 @default.
- W2075046091 cites W2054866291 @default.
- W2075046091 cites W2064928400 @default.
- W2075046091 cites W2065748070 @default.
- W2075046091 cites W2070543174 @default.
- W2075046091 cites W2070691875 @default.
- W2075046091 cites W2071583148 @default.
- W2075046091 cites W2073871141 @default.
- W2075046091 cites W2086942492 @default.
- W2075046091 cites W2088009882 @default.
- W2075046091 cites W2094317776 @default.
- W2075046091 cites W2100476052 @default.
- W2075046091 cites W2101942336 @default.
- W2075046091 cites W2158931004 @default.
- W2075046091 cites W2164992411 @default.
- W2075046091 doi "https://doi.org/10.1016/s0304-4203(97)00008-x" @default.
- W2075046091 hasPublicationYear "1997" @default.
- W2075046091 type Work @default.
- W2075046091 sameAs 2075046091 @default.
- W2075046091 citedByCount "23" @default.
- W2075046091 countsByYear W20750460912014 @default.
- W2075046091 countsByYear W20750460912016 @default.
- W2075046091 countsByYear W20750460912019 @default.
- W2075046091 countsByYear W20750460912021 @default.
- W2075046091 crossrefType "journal-article" @default.
- W2075046091 hasAuthorship W2075046091A5016341426 @default.
- W2075046091 hasAuthorship W2075046091A5052033048 @default.
- W2075046091 hasAuthorship W2075046091A5068824664 @default.
- W2075046091 hasConcept C111368507 @default.
- W2075046091 hasConcept C127313418 @default.
- W2075046091 hasConcept C147789679 @default.
- W2075046091 hasConcept C17525397 @default.
- W2075046091 hasConcept C178790620 @default.
- W2075046091 hasConcept C179104552 @default.
- W2075046091 hasConcept C184866935 @default.
- W2075046091 hasConcept C185592680 @default.
- W2075046091 hasConcept C197248824 @default.
- W2075046091 hasConcept C43617362 @default.
- W2075046091 hasConcept C51724486 @default.
- W2075046091 hasConcept C52859227 @default.
- W2075046091 hasConcept C544153396 @default.
- W2075046091 hasConcept C544778455 @default.
- W2075046091 hasConcept C73826308 @default.
- W2075046091 hasConcept C81557720 @default.
- W2075046091 hasConceptScore W2075046091C111368507 @default.
- W2075046091 hasConceptScore W2075046091C127313418 @default.
- W2075046091 hasConceptScore W2075046091C147789679 @default.
- W2075046091 hasConceptScore W2075046091C17525397 @default.
- W2075046091 hasConceptScore W2075046091C178790620 @default.
- W2075046091 hasConceptScore W2075046091C179104552 @default.
- W2075046091 hasConceptScore W2075046091C184866935 @default.
- W2075046091 hasConceptScore W2075046091C185592680 @default.
- W2075046091 hasConceptScore W2075046091C197248824 @default.
- W2075046091 hasConceptScore W2075046091C43617362 @default.
- W2075046091 hasConceptScore W2075046091C51724486 @default.
- W2075046091 hasConceptScore W2075046091C52859227 @default.
- W2075046091 hasConceptScore W2075046091C544153396 @default.
- W2075046091 hasConceptScore W2075046091C544778455 @default.
- W2075046091 hasConceptScore W2075046091C73826308 @default.
- W2075046091 hasConceptScore W2075046091C81557720 @default.
- W2075046091 hasIssue "1-2" @default.
- W2075046091 hasLocation W20750460911 @default.
- W2075046091 hasOpenAccess W2075046091 @default.
- W2075046091 hasPrimaryLocation W20750460911 @default.
- W2075046091 hasRelatedWork W1977008122 @default.
- W2075046091 hasRelatedWork W2002691539 @default.
- W2075046091 hasRelatedWork W2031992455 @default.
- W2075046091 hasRelatedWork W2066545624 @default.
- W2075046091 hasRelatedWork W2067518100 @default.
- W2075046091 hasRelatedWork W2083498870 @default.
- W2075046091 hasRelatedWork W2087138184 @default.
- W2075046091 hasRelatedWork W2100172952 @default.
- W2075046091 hasRelatedWork W278123735 @default.
- W2075046091 hasRelatedWork W757470175 @default.
- W2075046091 hasVolume "57" @default.
- W2075046091 isParatext "false" @default.
- W2075046091 isRetracted "false" @default.