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• W2075067327 abstract "This article presents a microscopic MR technique for imaging small mammalian fetuses in utero and in vivo which can be used as a tool for studying normal and abnormal development in small animal fetal models, for targeting in utero intervention in such models, and for following development serially. This new method is applied to a rat model of congenital diaphragmatic hernia (CDH). Pregnant Sprague-Dawley rats were fed nitrofen at 9.5 days postcoitus to induce CDH in the fetuses. The dams were imaged to identify fetuses with CDH for targeted in utero intervention, which consisted of fetal tracheal ligation. Following tracheal ligation, the fetuses were followed serially with our MR technique. For MR imaging, the dam was anesthetized with intramuscular ketamine and intraperitoneal pentobarbital. In utero imaging was performed on a 4 Tesla MRI system using a multislice, fast spin echo sequence with a long TR and short effective TE. These results were validated by examining individual fetuses postmortem using high-resolution MR and anatomic dissection. The in utero, in vivo MR technique is highly accurate for diagnosing CDH and following the effects of surgical intervention, and shows promise as a tool for the study of embryogenesis in small animal models. Magn Reson Med 44:331-335, 2000." @default.
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• W2075067327 date "2000-01-01" @default.
• W2075067327 modified "2023-10-18" @default.
• W2075067327 title "In vivo, in utero microscopic magnetic resonance imaging: Application in a rat model of diaphragmatic hernia" @default.
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• W2075067327 doi "https://doi.org/10.1002/1522-2594(200008)44:2<331::aid-mrm22>3.0.co;2-l" @default.
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