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- W2075124874 abstract "The ability of calli induced from leaf disks and immature seeds of Aralia cordata Thunb. to undergo somatic embryogenesis was investigated. Calli of 'Aichi-bozu' which were induced by leaf disk culture all produced somatic embryos on solid MS medium (Murashige and Skoog, 1962) modified as follows : NH4NO3 (1/4 of original) and 68.435 g·liter-1 (0.2 M) sucrose, supplemented with 1.0 mg·liter-1 2, 4-D. However, of the calli derived from the leaf disks of 'Iseshiro' and 'Murasaki-menoshiro' less than 65% regenerated embryos on all media. Of the calli derived from immature seeds (3.0 mm length) of 'Iseshiro' and 'Murasaki-menoshiro' 100% and 88.9%, respectivery, produced somatic embryos. In the former the calli were induced on the solid MS medium supplemented with 0.5mg or 1.0mg·liter-1 2, 4-D, whereas the latter contained 1.0mg·liter-1 2, 4-D. From these results, we found that in 'Aichi-bozu' the callus from leaf disks can produce somatic embryos at a very high frequency on MS medium with a low concentration of NH4NO3 and a high concentration of sucrose, supplemented with 2, 4-D, whereas, in 'Iseshiro' and 'Murasaki-menoshiro', the calli from immature seeds cultured on MS medium supplemented with 2, 4-D produced somatic embryos at high frequency. These somatic embryos grew into complete plants." @default.
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- W2075124874 date "1998-01-01" @default.
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- W2075124874 title "Somatic Embryogenesis from Leaf Disks and Immature Seeds of Aralia cordata Thunb." @default.
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- W2075124874 doi "https://doi.org/10.2503/jjshs.67.81" @default.
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