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- W2075143991 abstract "In situ hybridization was used to detect messenger RNA (mRNA) in a variety of rat tissues which were fixed in formalin either immediately after death or after a 24 h period of storage at 5°C. A synthetic polydeoxythymidine [poly d(T)] oligonucleotide probe was used to demonstrate poiyadenylated [poly (A)] mRNA in the small intestine, pancreas, liver, cerebellum, and pituitary. Of these tissues, only the liver showed a small reproducible reduction in hybridization signal following delayed fixation. Synthetic oligonucleotide probes complementary to albumin and pro-opiomelanocortin (POMC) mRNAs were hybridized to liver and pituitary, respectively. There was no significant reduction in hybridization signal in post-mortem tissues. The results suggest that some mRNAs may be remarkably stable under certain postmortem conditions and this should encourage the wider application of in situ hybridization techniques to post-mortem material." @default.
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- W2075143991 title "In situ Hybridization demonstrates the stability of mRNA in post-mortem rat tissues" @default.
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- W2075143991 doi "https://doi.org/10.1002/path.1711680112" @default.
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