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- W2075190038 abstract "Abstract Ceramides were subjected to direct separation and structural analysis by high performance liquid chromatography after derivatization with benzoyl and p ‐nitrobenzoyl chloride. The structures of benzoyl and p ‐nitrobenzoyl derivatives of a representative ceramide, N‐heptadecanoyl D‐sphingosine, were fully characterized by mass spectrometry. Excellent separation of molecular species of ceramides was achieved with a 10‐μ LiChrosorb RP18 column by isocratic elution. Both the acyl group and the long‐chain base moiety were separated on this reverse‐phase column. The method has been used to determine quantitatively molecular species of nonhydroxy ceramides derived from bovine brain and egg yolk. Comparison of gas liquid chromatographic and high performance liquid chromatographic assays of intact ceramides was made." @default.
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- W2075190038 title "Separation of molecular species of ceramides as benzoyl andp-nitrobenzoyl derivatives by high performance liquid chromatography" @default.
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