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• W2075283315 abstract "The promoter regions of nifA and nifH genes of Herbaspirillom seropedicae have been isolated and investigated, and the nifA promoter has been characterized in detail. Both regions contain a −24−12 type promoter and NifA-binding sites; the nifA promoter also has an NtrC-binding site. The nifA expression is activated by NtrC and repressed by ammonium ions but not by oxygen. When the NtrC-binding site was deleted nifA expression was NifA-dependent. The native NifA protein of H. seropedicae was unable to activate nifH expression in Escherichia coli whereas a truncated protein, lacking the N-terminal domain was able to activate nifH expression in the absence of oxygen and presence of iron, indicating that the N-terminal domain regulates NifA activity. The truncated protein also activates nifH in Azospirillum brasilense in the presence or absence of ammonium ions. This suggests that the N-terminal domain senses the nitrogen status of the cell." @default.
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• W2075283315 date "1997-05-01" @default.
• W2075283315 modified "2023-10-17" @default.
• W2075283315 title "Structural organization and regulation of the nif genes of Herbaspirillum seropedicae" @default.
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• W2075283315 doi "https://doi.org/10.1016/s0038-0717(96)00206-4" @default.
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