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- W2075294080 abstract "Abstract Langmuir–Blodgett (LB) film deposition technique was employed for the immobilization of organophosphorus hydrolase (OPH). OPH enzyme was covalently bonded to a fluorescent probe, fluorescein isothiocyanate (FITC), and used as a biological recognition element. Under optimal experimental conditions, OPH monolayers were deposited onto the surface of silanized quartz slides as LB film and utilized as a bioassay for the detection of paraoxon. Two different methods were employed for detection of paraoxon: the fluorescence quenching of the fluorescence probe (FITC) covalently bonded to OPH and the UV–vis absorption spectrum of the paraoxon hydrolysis product. The UV–vis absorption measurement demonstrated a linear relationship between the absorbance at 400 nm and the concentration of paraoxon solutions over the range of 1.0 × 10−7–1.0 × 10−5 M (0.27–27 ppm). By observing the FITC fluorescence quenching, the concentration of paraoxon can be detected as low as 10−9 M (S/N = 3). The research described herein showed that the LB film bioassay had high sensitivity, rapid response time and good reproducibility." @default.
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- W2075294080 date "2004-12-01" @default.
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- W2075294080 title "Detection of paraoxon by immobilized organophosphorus hydrolase in a Langmuir–Blodgett film" @default.
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- W2075294080 doi "https://doi.org/10.1016/j.colsurfa.2004.01.043" @default.
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