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• W2075308132 abstract "In our recent article In vivo evolution of metabolic pathways by homeologous recombination in mitotic cells we proposed a useful alternative to directed evolution methods that permits the generation of yeast cell libraries containing recombinant metabolic pathways from counterpart genes. The methodology was applied to generate single mosaic genes and intragenic mosaic pathways. We used flavonoid metabolism genes as a working model to assembly and express evolved pathways in DNA repair deficient cells. The present commentary revises the principles of gene and pathway mosaicism and explores the scope and perspectives of our results as an additional tool for synthetic biology." @default.
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• W2075308132 date "2014-10-30" @default.
• W2075308132 modified "2023-09-27" @default.
• W2075308132 title "In vivo evolution of metabolic pathways: Assembling old parts to build novel and functional structures" @default.
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• W2075308132 doi "https://doi.org/10.4161/bioe.34347" @default.
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