FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2075328327> ?p ?o ?g. }

• W2075328327 endingPage "2940" @default.
• W2075328327 startingPage "2927" @default.
• W2075328327 abstract "Cells, scaffolds, and growth factors are the three main factors for creating a stem-cell-based tissue-engineered construct, but the interactions between three factors are not very clear. We hereby explored the interactions between rat-adipose-derived stromal cells (rASCs), recombinant human bone morphogenetic protein-2 (rhBMP-2), and beta-tricalcium phosphate (beta-TCP) to provide evidence for their application in bone tissue engineering by evaluating the protein adsorption of beta-TCP, the cell attachment, alkaline phosphatase (ALP) activity/protein, osteocalcin (OCN) content, mineral formation, calcium content, phosphonium content, cell vitality, gene expression, and implantation in the backs of severe combined immunodeficient mice of rhBMP-2 preinducing rASCs seeded onto beta-TCP. The results showed that beta-TCP could adsorb the proteins from the media. The attachment, proliferation, and osteogenic properties of rASCs were supported by beta-TCP, as revealed using scanning electron microscopy. Compared with rASCs cultured on the culture plate, rASCs cultured on beta-TCP had significantly higher ALP activity/protein, OCN content, and mineral formation. These values for rASCs cultured on beta-TCP with rhBMP-2 increased most significantly. The rhBMP-2 significantly increased the calcium content, phosphonium content, and ALP, type I collagen, and OCN mRNA levels of rASCs cultured on beta-TCP. The methylthiazol tetrazolium method revealed that the vitality of rASCs cultured on beta-TCP with or without rhBMP-2 for 4, 7, and 28 days in vitro was insignificantly different. After 8 and 12 weeks of implantation, each group displayed increased bone formation over the 12-week period. The percentage of the new bone formed areas for beta-TCP/rhBMP-2 and beta-TCP was not significantly different. This value for rASCs/beta-TCP construct was significantly higher than that for beta-TCP group, but the maximal and robust bone formation was presented in rASCs/beta-TCP with rhBMP-2. The results implied that stem cells existed in adult rat adipose tissue. beta-TCP could adsorb rhBMP-2 from the media and had osteoinductivity when alone implanted in the back of severe combined immunodeficient mice. beta-TCP was also sufficient to trigger the differentiation of rASCs toward an osteoblastic phenotype without the addition of osteogenic factor. The rhBMP-2 could better sufficiently induce osteogenic differentiation of rASCs seeded onto beta-TCP. The rASCs and rhBMP-2 could promote the dissolution of beta-TCP to provide Ca2+ and PO4(3-) needed for bone formation. The interactions between the three factors could provide an optimizing microenvironment for osteogenic differentiation of rASCs, and this might be essential for sufficient and timely bone formation in vivo. This study may provide insight into the clinical repair of bone defect with ASCs+beta-TCP+rhBMP-2 construct." @default.
• W2075328327 created "2016-06-24" @default.
• W2075328327 creator A5014235931 @default.
• W2075328327 creator A5020116913 @default.
• W2075328327 creator A5021343520 @default.
• W2075328327 creator A5025256775 @default.
• W2075328327 creator A5034537559 @default.
• W2075328327 creator A5053995579 @default.
• W2075328327 creator A5090380548 @default.
• W2075328327 date "2010-09-01" @default.
• W2075328327 modified "2023-09-27" @default.
• W2075328327 title "The Interactions Between Rat-Adipose-Derived Stromal Cells, Recombinant Human Bone Morphogenetic Protein-2, and Beta-Tricalcium Phosphate Play an Important Role in Bone Tissue Engineering" @default.
• W2075328327 cites W1537411924 @default.
• W2075328327 cites W1970643062 @default.
• W2075328327 cites W1973006978 @default.
• W2075328327 cites W1978405687 @default.
• W2075328327 cites W1984255682 @default.
• W2075328327 cites W1992902310 @default.
• W2075328327 cites W1997797226 @default.
• W2075328327 cites W2003649275 @default.
• W2075328327 cites W2006549340 @default.
• W2075328327 cites W2007564570 @default.
• W2075328327 cites W2029338429 @default.
• W2075328327 cites W2029348465 @default.
• W2075328327 cites W2032740145 @default.
• W2075328327 cites W2033391292 @default.
• W2075328327 cites W2038467210 @default.
• W2075328327 cites W2055220860 @default.
• W2075328327 cites W2056264620 @default.
• W2075328327 cites W2057626920 @default.
• W2075328327 cites W2063095947 @default.
• W2075328327 cites W2065739747 @default.
• W2075328327 cites W2068812118 @default.
• W2075328327 cites W2072519595 @default.
• W2075328327 cites W2079043866 @default.
• W2075328327 cites W2079619188 @default.
• W2075328327 cites W2081593181 @default.
• W2075328327 cites W2086194573 @default.
• W2075328327 cites W2089779401 @default.
• W2075328327 cites W2118458879 @default.
• W2075328327 cites W2143245418 @default.
• W2075328327 cites W2144142985 @default.
• W2075328327 cites W2149301498 @default.
• W2075328327 cites W2160935729 @default.
• W2075328327 cites W2168745560 @default.
• W2075328327 cites W2173497140 @default.
• W2075328327 cites W2225041880 @default.
• W2075328327 cites W4293411852 @default.
• W2075328327 doi "https://doi.org/10.1089/ten.tea.2010.0018" @default.
• W2075328327 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20486786" @default.
• W2075328327 hasPublicationYear "2010" @default.
• W2075328327 type Work @default.
• W2075328327 sameAs 2075328327 @default.
• W2075328327 citedByCount "31" @default.
• W2075328327 countsByYear W20753283272012 @default.
• W2075328327 countsByYear W20753283272013 @default.
• W2075328327 countsByYear W20753283272014 @default.
• W2075328327 countsByYear W20753283272015 @default.
• W2075328327 countsByYear W20753283272016 @default.
• W2075328327 countsByYear W20753283272017 @default.
• W2075328327 countsByYear W20753283272018 @default.
• W2075328327 countsByYear W20753283272019 @default.
• W2075328327 countsByYear W20753283272020 @default.
• W2075328327 countsByYear W20753283272021 @default.
• W2075328327 countsByYear W20753283272023 @default.
• W2075328327 crossrefType "journal-article" @default.
• W2075328327 hasAuthorship W2075328327A5014235931 @default.
• W2075328327 hasAuthorship W2075328327A5020116913 @default.
• W2075328327 hasAuthorship W2075328327A5021343520 @default.
• W2075328327 hasAuthorship W2075328327A5025256775 @default.
• W2075328327 hasAuthorship W2075328327A5034537559 @default.
• W2075328327 hasAuthorship W2075328327A5053995579 @default.
• W2075328327 hasAuthorship W2075328327A5090380548 @default.
• W2075328327 hasConcept C126322002 @default.
• W2075328327 hasConcept C131075544 @default.
• W2075328327 hasConcept C136229726 @default.
• W2075328327 hasConcept C160160445 @default.
• W2075328327 hasConcept C16930146 @default.
• W2075328327 hasConcept C171089720 @default.
• W2075328327 hasConcept C178790620 @default.
• W2075328327 hasConcept C181199279 @default.
• W2075328327 hasConcept C185592680 @default.
• W2075328327 hasConcept C202751555 @default.
• W2075328327 hasConcept C30352789 @default.
• W2075328327 hasConcept C49892992 @default.
• W2075328327 hasConcept C519063684 @default.
• W2075328327 hasConcept C55493867 @default.
• W2075328327 hasConcept C71924100 @default.
• W2075328327 hasConcept C86803240 @default.
• W2075328327 hasConcept C95444343 @default.
• W2075328327 hasConceptScore W2075328327C126322002 @default.
• W2075328327 hasConceptScore W2075328327C131075544 @default.
• W2075328327 hasConceptScore W2075328327C136229726 @default.
• W2075328327 hasConceptScore W2075328327C160160445 @default.
• W2075328327 hasConceptScore W2075328327C16930146 @default.
• W2075328327 hasConceptScore W2075328327C171089720 @default.
• W2075328327 hasConceptScore W2075328327C178790620 @default.
• W2075328327 hasConceptScore W2075328327C181199279 @default.

• next