Matches in SemOpenAlex for { <https://semopenalex.org/work/W2075387141> ?p ?o ?g. }
- W2075387141 endingPage "116" @default.
- W2075387141 startingPage "109" @default.
- W2075387141 abstract "The beta-1,4-mannanase 5C gene (man5C) of Vibrio sp. strain MA-138 was cloned and expressed in Escherichia coli. The man5C gene consisted of 2,010 bp nucleotides encoding a protein of 669 amino acids with a predicted molecular weight of 76,309. beta-1,4-Mannanase (Man5C) is a modular enzyme composed of a catalytic module belonging to glycoside hydrolase family 5, a linker region, and a putative carbohydrate-binding module (CBM) belonging to family 27. Recombinant Man5C exhibited maximal activity at 50 degrees C at pH 7.0, and it had a K(m) of 0.6 mg ml(-1) and a V(max) of 556.2 micromol min(-1) mumol(-1) for glucomannan. Binding studies revealed that the C-terminal putative CBM27 had the ability to bind soluble beta-mannans and contributed to increasing the rate of depolymerization by binding to the polymeric substrate. Man5C of Vibrio sp. MA-138 is the first non-extremophile enzyme to be identified as a beta-mannanase possessing CBM27." @default.
- W2075387141 created "2016-06-24" @default.
- W2075387141 creator A5000001556 @default.
- W2075387141 creator A5006228837 @default.
- W2075387141 creator A5047234832 @default.
- W2075387141 creator A5072430122 @default.
- W2075387141 creator A5081424218 @default.
- W2075387141 creator A5088549917 @default.
- W2075387141 date "2009-01-23" @default.
- W2075387141 modified "2023-10-01" @default.
- W2075387141 title "Cloning and Characterization of a β-1,4-Mannanase 5C Possessing a Family 27 Carbohydrate-Binding Module from a Marine Bacterium,<i>Vibrio</i>sp. Strain MA-138" @default.
- W2075387141 cites W1491472429 @default.
- W2075387141 cites W1507396609 @default.
- W2075387141 cites W1529201898 @default.
- W2075387141 cites W1568028271 @default.
- W2075387141 cites W1775749144 @default.
- W2075387141 cites W1793573788 @default.
- W2075387141 cites W1830745998 @default.
- W2075387141 cites W1880992470 @default.
- W2075387141 cites W1899953143 @default.
- W2075387141 cites W1944827779 @default.
- W2075387141 cites W1964149834 @default.
- W2075387141 cites W1970520418 @default.
- W2075387141 cites W1975588657 @default.
- W2075387141 cites W1981721023 @default.
- W2075387141 cites W1982112062 @default.
- W2075387141 cites W1985003823 @default.
- W2075387141 cites W1985336462 @default.
- W2075387141 cites W1998788198 @default.
- W2075387141 cites W2008922250 @default.
- W2075387141 cites W2010312364 @default.
- W2075387141 cites W2010759469 @default.
- W2075387141 cites W2014276356 @default.
- W2075387141 cites W2016001814 @default.
- W2075387141 cites W2023391968 @default.
- W2075387141 cites W2042515734 @default.
- W2075387141 cites W2043992754 @default.
- W2075387141 cites W2044116389 @default.
- W2075387141 cites W2050119948 @default.
- W2075387141 cites W2055043387 @default.
- W2075387141 cites W2077250439 @default.
- W2075387141 cites W2082891495 @default.
- W2075387141 cites W2084720501 @default.
- W2075387141 cites W2100115720 @default.
- W2075387141 cites W2100837269 @default.
- W2075387141 cites W2104248189 @default.
- W2075387141 cites W2113964973 @default.
- W2075387141 cites W2114618967 @default.
- W2075387141 cites W2114624600 @default.
- W2075387141 cites W2126036775 @default.
- W2075387141 cites W2126487177 @default.
- W2075387141 cites W2130959801 @default.
- W2075387141 cites W2137450588 @default.
- W2075387141 cites W2138913088 @default.
- W2075387141 cites W2151331848 @default.
- W2075387141 cites W2152908973 @default.
- W2075387141 cites W2157913345 @default.
- W2075387141 cites W2171091522 @default.
- W2075387141 cites W2313553182 @default.
- W2075387141 cites W2315084059 @default.
- W2075387141 cites W2328867816 @default.
- W2075387141 cites W397395867 @default.
- W2075387141 cites W911357984 @default.
- W2075387141 doi "https://doi.org/10.1271/bbb.80521" @default.
- W2075387141 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19129644" @default.
- W2075387141 hasPublicationYear "2009" @default.
- W2075387141 type Work @default.
- W2075387141 sameAs 2075387141 @default.
- W2075387141 citedByCount "30" @default.
- W2075387141 countsByYear W20753871412012 @default.
- W2075387141 countsByYear W20753871412013 @default.
- W2075387141 countsByYear W20753871412014 @default.
- W2075387141 countsByYear W20753871412015 @default.
- W2075387141 countsByYear W20753871412016 @default.
- W2075387141 countsByYear W20753871412017 @default.
- W2075387141 countsByYear W20753871412018 @default.
- W2075387141 countsByYear W20753871412019 @default.
- W2075387141 countsByYear W20753871412020 @default.
- W2075387141 countsByYear W20753871412021 @default.
- W2075387141 countsByYear W20753871412022 @default.
- W2075387141 crossrefType "journal-article" @default.
- W2075387141 hasAuthorship W2075387141A5000001556 @default.
- W2075387141 hasAuthorship W2075387141A5006228837 @default.
- W2075387141 hasAuthorship W2075387141A5047234832 @default.
- W2075387141 hasAuthorship W2075387141A5072430122 @default.
- W2075387141 hasAuthorship W2075387141A5081424218 @default.
- W2075387141 hasAuthorship W2075387141A5088549917 @default.
- W2075387141 hasBestOaLocation W20753871411 @default.
- W2075387141 hasConcept C104317684 @default.
- W2075387141 hasConcept C105702510 @default.
- W2075387141 hasConcept C167625842 @default.
- W2075387141 hasConcept C181199279 @default.
- W2075387141 hasConcept C185592680 @default.
- W2075387141 hasConcept C196032511 @default.
- W2075387141 hasConcept C19924922 @default.
- W2075387141 hasConcept C2776154503 @default.
- W2075387141 hasConcept C2776519085 @default.
- W2075387141 hasConcept C2778022156 @default.