Matches in SemOpenAlex for { <https://semopenalex.org/work/W2075480964> ?p ?o ?g. }
- W2075480964 endingPage "521" @default.
- W2075480964 startingPage "513" @default.
- W2075480964 abstract "Rhodanese is a multifunctional, sulfur transferase that catalyzes the detoxification of cyanide by sulphuration in a double displacement (ping pong) mechanistic reaction. In the present study, small-insert metagenomic library from soil sample collected from Ladakh (3,000–3,600 m.a.s.l) in northwestern Himalayas, India was constructed. Function-driven screening of ~8,500 colonies led to the isolation of one esterase-positive clone (clone-est) harboring 2.43 kb insert. Sequence analysis of the insert identified two ORF’s, phosM encoding phosphoesterase and rodM encoding rhodanese. The 800 bp rodM gene encoded a polypeptide of 227 amino acids (RodM). The RodM showed maximum homology with the rhodanese-like protein from Cyanobacterium synechococcus species with a score identity of only 51 %. Putative 3D structure of RodM developed by homology modeling resembles to homodimeric protein of SUD sulfur transferase of Wolinella succinogenes with properly structured active-site cysteine (Cys) residue. Rhodanese has been reported from few culturable microorganisms." @default.
- W2075480964 created "2016-06-24" @default.
- W2075480964 creator A5017280088 @default.
- W2075480964 creator A5056177485 @default.
- W2075480964 creator A5067103538 @default.
- W2075480964 creator A5072064951 @default.
- W2075480964 date "2014-10-02" @default.
- W2075480964 modified "2023-10-06" @default.
- W2075480964 title "Molecular cloning of rhodanese gene from soil metagenome of cold desert of North-West Himalayas: sequence and structural features of the rhodanese enzyme" @default.
- W2075480964 cites W1602125069 @default.
- W2075480964 cites W1964576473 @default.
- W2075480964 cites W1965937322 @default.
- W2075480964 cites W1966110999 @default.
- W2075480964 cites W1968413588 @default.
- W2075480964 cites W1974770657 @default.
- W2075480964 cites W2025036474 @default.
- W2075480964 cites W2025294451 @default.
- W2075480964 cites W2032648454 @default.
- W2075480964 cites W2041586491 @default.
- W2075480964 cites W2057902040 @default.
- W2075480964 cites W2060259273 @default.
- W2075480964 cites W2061750017 @default.
- W2075480964 cites W2065898689 @default.
- W2075480964 cites W2069795071 @default.
- W2075480964 cites W2071092023 @default.
- W2075480964 cites W2072096428 @default.
- W2075480964 cites W2083155681 @default.
- W2075480964 cites W2090660578 @default.
- W2075480964 cites W2097706568 @default.
- W2075480964 cites W2097943773 @default.
- W2075480964 cites W2100112052 @default.
- W2075480964 cites W2105650917 @default.
- W2075480964 cites W2105954277 @default.
- W2075480964 cites W2106882534 @default.
- W2075480964 cites W2115953467 @default.
- W2075480964 cites W2125121305 @default.
- W2075480964 cites W2127347907 @default.
- W2075480964 cites W2144215775 @default.
- W2075480964 cites W2153823136 @default.
- W2075480964 cites W2158714788 @default.
- W2075480964 cites W2265639645 @default.
- W2075480964 cites W4211254727 @default.
- W2075480964 doi "https://doi.org/10.1007/s13205-014-0249-2" @default.
- W2075480964 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4522728" @default.
- W2075480964 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28324556" @default.
- W2075480964 hasPublicationYear "2014" @default.
- W2075480964 type Work @default.
- W2075480964 sameAs 2075480964 @default.
- W2075480964 citedByCount "6" @default.
- W2075480964 countsByYear W20754809642017 @default.
- W2075480964 countsByYear W20754809642018 @default.
- W2075480964 countsByYear W20754809642019 @default.
- W2075480964 countsByYear W20754809642022 @default.
- W2075480964 countsByYear W20754809642023 @default.
- W2075480964 crossrefType "journal-article" @default.
- W2075480964 hasAuthorship W2075480964A5017280088 @default.
- W2075480964 hasAuthorship W2075480964A5056177485 @default.
- W2075480964 hasAuthorship W2075480964A5067103538 @default.
- W2075480964 hasAuthorship W2075480964A5072064951 @default.
- W2075480964 hasBestOaLocation W20754809641 @default.
- W2075480964 hasConcept C104317684 @default.
- W2075480964 hasConcept C15151743 @default.
- W2075480964 hasConcept C165525559 @default.
- W2075480964 hasConcept C167625842 @default.
- W2075480964 hasConcept C169627665 @default.
- W2075480964 hasConcept C181199279 @default.
- W2075480964 hasConcept C2779033446 @default.
- W2075480964 hasConcept C2779201268 @default.
- W2075480964 hasConcept C2780637352 @default.
- W2075480964 hasConcept C54355233 @default.
- W2075480964 hasConcept C55493867 @default.
- W2075480964 hasConcept C86803240 @default.
- W2075480964 hasConceptScore W2075480964C104317684 @default.
- W2075480964 hasConceptScore W2075480964C15151743 @default.
- W2075480964 hasConceptScore W2075480964C165525559 @default.
- W2075480964 hasConceptScore W2075480964C167625842 @default.
- W2075480964 hasConceptScore W2075480964C169627665 @default.
- W2075480964 hasConceptScore W2075480964C181199279 @default.
- W2075480964 hasConceptScore W2075480964C2779033446 @default.
- W2075480964 hasConceptScore W2075480964C2779201268 @default.
- W2075480964 hasConceptScore W2075480964C2780637352 @default.
- W2075480964 hasConceptScore W2075480964C54355233 @default.
- W2075480964 hasConceptScore W2075480964C55493867 @default.
- W2075480964 hasConceptScore W2075480964C86803240 @default.
- W2075480964 hasIssue "4" @default.
- W2075480964 hasLocation W20754809641 @default.
- W2075480964 hasLocation W20754809642 @default.
- W2075480964 hasLocation W20754809643 @default.
- W2075480964 hasLocation W20754809644 @default.
- W2075480964 hasOpenAccess W2075480964 @default.
- W2075480964 hasPrimaryLocation W20754809641 @default.
- W2075480964 hasRelatedWork W1480777598 @default.
- W2075480964 hasRelatedWork W1966110036 @default.
- W2075480964 hasRelatedWork W1973200129 @default.
- W2075480964 hasRelatedWork W1991471459 @default.
- W2075480964 hasRelatedWork W2040571417 @default.
- W2075480964 hasRelatedWork W2045313050 @default.
- W2075480964 hasRelatedWork W2074557265 @default.