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- W2075491301 abstract "Detecting impacts of organophosphorus biocides (OP) is facilitated by analysing biomarkers - biological responses to environmental insults. Understanding is hampered by studying biomarkers in isolation at different levels of biological response and limited work on ecologically-important species. We tested the relevance of esterases as biomarkers of OP-exposure in limpets (Patella vulgata), abundant prosobranchs that structure the assemblages on rocky shores through their grazing. We characterized esterases in haemolymph and tissue, and quantified their dose-dependent inhibition by chlorfenvinphos (0.1-3.0 mM) in vitro. To determine whether esterases are useful biomarkers we exposed limpets to chlorfenvinphos (0-10 μg L(-1)). Despite reduced tenacity (ability to stick to a surface) and haemocyte-viability, esterases remained unaffected. Tenacity was reduced by >50% at 5 μg L(-1) and by 95% at 10 μg L(-1), whilst haemocyte-viability was more sensitive with >40% reductions at concentrations of 0.5 μg L(-1) and above. We discuss results in relation to linking sub-lethal and ecological impacts at contaminated sites." @default.
- W2075491301 created "2016-06-24" @default.
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- W2075491301 date "2015-08-01" @default.
- W2075491301 modified "2023-09-23" @default.
- W2075491301 title "Organophosphorous biocides reduce tenacity and cellular viability but not esterase activities in a non-target prosobranch (limpet)" @default.
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- W2075491301 doi "https://doi.org/10.1016/j.envpol.2015.04.001" @default.
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