Matches in SemOpenAlex for { <https://semopenalex.org/work/W2075493224> ?p ?o ?g. }
- W2075493224 endingPage "820" @default.
- W2075493224 startingPage "806" @default.
- W2075493224 abstract "ABSTRACT Human cytomegalovirus infections involve the extensive modification of host cell pathways, including cell cycle control, the regulation of the DNA damage response, and averting promyelocytic leukemia (PML)-mediated antiviral responses. The UL35 gene from human cytomegalovirus is important for viral gene expression and efficient replication and encodes two proteins, UL35 and UL35a, whose mechanism of action is not well understood. Here, affinity purification coupled with mass spectrometry was used to identify previously unknown human cellular targets of UL35 and UL35a. We demonstrate that both viral proteins interact with the ubiquitin-specific protease USP7, and that UL35 expression can alter USP7 subcellular localization. In addition, UL35 (but not UL35a) was found to associate with three components of the Cul4 DCAF1 E3 ubiquitin ligase complex (DCAF1, DDB1, and DDA1) previously shown to be targeted by the HIV-1 Vpr protein. The coimmunoprecipitation and immunofluorescence microscopy of DCAF1 mutants revealed that the C-terminal region of DCAF1 is required for association with UL35 and mediates the dramatic relocalization of DCAF1 to UL35 nuclear bodies, which also contain conjugated ubiquitin. As previously reported for the Vpr-DCAF1 interaction, UL35 (but not UL35a) expression resulted in the accumulation of cells in the G 2 phase of the cell cycle, which is typical of a DNA damage response, and activated the G 2 checkpoint in a DCAF1-dependent manner. In addition, UL35 (but not UL35a) induced γ-H2AX and 53BP1 foci, indicating the activation of DNA damage and repair responses. Therefore, the identified interactions suggest that UL35 can contribute to viral replication through the manipulation of host responses." @default.
- W2075493224 created "2016-06-24" @default.
- W2075493224 creator A5004746373 @default.
- W2075493224 creator A5021397625 @default.
- W2075493224 creator A5039562464 @default.
- W2075493224 creator A5040453565 @default.
- W2075493224 creator A5042478049 @default.
- W2075493224 creator A5060756067 @default.
- W2075493224 creator A5078364744 @default.
- W2075493224 date "2012-01-15" @default.
- W2075493224 modified "2023-10-11" @default.
- W2075493224 title "Proteomic Profiling of the Human Cytomegalovirus UL35 Gene Products Reveals a Role for UL35 in the DNA Repair Response" @default.
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