Matches in SemOpenAlex for { <https://semopenalex.org/work/W2075495253> ?p ?o ?g. }
- W2075495253 endingPage "2358" @default.
- W2075495253 startingPage "2352" @default.
- W2075495253 abstract "Membrane proteins are extremely challenging to produce in sufficient quantities for biochemical and structural analysis and there is a growing demand for solutions to this problem. In this study we attempted to improve expression of two difficult-to-express coding sequences (araH and narK) for membrane transporters. For both coding sequences, synonymous codon substitutions in the region adjacent to the AUG start led to significant improvements in expression, whereas multi-parameter sequence optimization of codons throughout the coding sequence failed. We conclude that coding sequences can be re-wired for high-level protein expression by selective engineering of the 5′ coding sequence with synonymous codons, thus circumventing the need to consider whole sequence optimization." @default.
- W2075495253 created "2016-06-24" @default.
- W2075495253 creator A5007682824 @default.
- W2075495253 creator A5031393513 @default.
- W2075495253 creator A5041437001 @default.
- W2075495253 creator A5051008473 @default.
- W2075495253 creator A5066814196 @default.
- W2075495253 creator A5072454967 @default.
- W2075495253 date "2013-06-12" @default.
- W2075495253 modified "2023-10-10" @default.
- W2075495253 title "Improved production of membrane proteins in <i>Escherichia coli</i> by selective codon substitutions" @default.
- W2075495253 cites W1692054786 @default.
- W2075495253 cites W1971954298 @default.
- W2075495253 cites W1978140423 @default.
- W2075495253 cites W1990621556 @default.
- W2075495253 cites W1996423252 @default.
- W2075495253 cites W2006492629 @default.
- W2075495253 cites W2006719424 @default.
- W2075495253 cites W2007715970 @default.
- W2075495253 cites W2008365039 @default.
- W2075495253 cites W2014776147 @default.
- W2075495253 cites W2015214828 @default.
- W2075495253 cites W2025481401 @default.
- W2075495253 cites W2031853427 @default.
- W2075495253 cites W2042700625 @default.
- W2075495253 cites W2051242197 @default.
- W2075495253 cites W2058658881 @default.
- W2075495253 cites W2064050626 @default.
- W2075495253 cites W2065035185 @default.
- W2075495253 cites W2066829062 @default.
- W2075495253 cites W2072499238 @default.
- W2075495253 cites W2085116840 @default.
- W2075495253 cites W2085522899 @default.
- W2075495253 cites W2092035637 @default.
- W2075495253 cites W2092145286 @default.
- W2075495253 cites W2092247420 @default.
- W2075495253 cites W2099435024 @default.
- W2075495253 cites W2115728811 @default.
- W2075495253 cites W2120498503 @default.
- W2075495253 cites W2125071576 @default.
- W2075495253 cites W2165439827 @default.
- W2075495253 cites W2165991512 @default.
- W2075495253 cites W2168471605 @default.
- W2075495253 cites W2170602689 @default.
- W2075495253 doi "https://doi.org/10.1016/j.febslet.2013.05.063" @default.
- W2075495253 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23769986" @default.
- W2075495253 hasPublicationYear "2013" @default.
- W2075495253 type Work @default.
- W2075495253 sameAs 2075495253 @default.
- W2075495253 citedByCount "37" @default.
- W2075495253 countsByYear W20754952532013 @default.
- W2075495253 countsByYear W20754952532014 @default.
- W2075495253 countsByYear W20754952532015 @default.
- W2075495253 countsByYear W20754952532016 @default.
- W2075495253 countsByYear W20754952532017 @default.
- W2075495253 countsByYear W20754952532018 @default.
- W2075495253 countsByYear W20754952532019 @default.
- W2075495253 countsByYear W20754952532020 @default.
- W2075495253 countsByYear W20754952532021 @default.
- W2075495253 countsByYear W20754952532022 @default.
- W2075495253 crossrefType "journal-article" @default.
- W2075495253 hasAuthorship W2075495253A5007682824 @default.
- W2075495253 hasAuthorship W2075495253A5031393513 @default.
- W2075495253 hasAuthorship W2075495253A5041437001 @default.
- W2075495253 hasAuthorship W2075495253A5051008473 @default.
- W2075495253 hasAuthorship W2075495253A5066814196 @default.
- W2075495253 hasAuthorship W2075495253A5072454967 @default.
- W2075495253 hasBestOaLocation W20754952531 @default.
- W2075495253 hasConcept C104317684 @default.
- W2075495253 hasConcept C105795698 @default.
- W2075495253 hasConcept C141231307 @default.
- W2075495253 hasConcept C176990463 @default.
- W2075495253 hasConcept C179518139 @default.
- W2075495253 hasConcept C20580545 @default.
- W2075495253 hasConcept C2778112365 @default.
- W2075495253 hasConcept C3017666073 @default.
- W2075495253 hasConcept C33923547 @default.
- W2075495253 hasConcept C54355233 @default.
- W2075495253 hasConcept C547475151 @default.
- W2075495253 hasConcept C70721500 @default.
- W2075495253 hasConcept C86803240 @default.
- W2075495253 hasConcept C87253356 @default.
- W2075495253 hasConcept C91779695 @default.
- W2075495253 hasConceptScore W2075495253C104317684 @default.
- W2075495253 hasConceptScore W2075495253C105795698 @default.
- W2075495253 hasConceptScore W2075495253C141231307 @default.
- W2075495253 hasConceptScore W2075495253C176990463 @default.
- W2075495253 hasConceptScore W2075495253C179518139 @default.
- W2075495253 hasConceptScore W2075495253C20580545 @default.
- W2075495253 hasConceptScore W2075495253C2778112365 @default.
- W2075495253 hasConceptScore W2075495253C3017666073 @default.
- W2075495253 hasConceptScore W2075495253C33923547 @default.
- W2075495253 hasConceptScore W2075495253C54355233 @default.
- W2075495253 hasConceptScore W2075495253C547475151 @default.
- W2075495253 hasConceptScore W2075495253C70721500 @default.
- W2075495253 hasConceptScore W2075495253C86803240 @default.
- W2075495253 hasConceptScore W2075495253C87253356 @default.
- W2075495253 hasConceptScore W2075495253C91779695 @default.