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- W2075564560 abstract "A commercially available 27 MHz quartz crystal microbalance (QCM) has been employed to investigate the in-situ binding of avidin to carbodiimide hydrochloride/N-hydroxysuccinimide activated carboxylic acid modified QCM electrodes, and the subsequent binding of single-stranded 21- and 30-mer biotinylated DNA [single-stranded (ss) biotin-DNA]. The interaction between surface-immobilized ss biotin-DNA and complementary 21-mer DNA in solution was also examined. Each step was followed by monitoring changes in the QCM frequency with time. Hybridization of immobilized ss biotin-DNA with complementary 21-mer DNA oligonucleotides revealed an immobilized DNA-probe (ss biotin-DNA) to hybridized-DNA target ratio of 1:1. The 27 MHz QCM is shown to be approximately 10 times more sensitive than the 9 MHz QCMs commonly used in sensing experiments." @default.
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- W2075564560 date "1998-03-01" @default.
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- W2075564560 title "In-situ measurement of DNA immobilization and hybridization using a 27MHz quartz crystal microbalance" @default.
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- W2075564560 doi "https://doi.org/10.1016/s0927-7765(97)00067-2" @default.
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