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- W2075606418 abstract "ABSTRACT The complete sequence of the virulence plasmid pMT1 of Yersinia pestis KIM5 revealed a region homologous to the plasmid partition ( par ) region of the P7 plasmid prophage of Escherichia coli . The essential genes parA and parB and the downstream partition site gene, parS , are highly conserved in sequence and organization. The pMT1 parS site and the parA-parB operon were separately inserted into vectors that could be maintained in E. coli . A mini-P1 vector containing pMT1 parS was stably maintained when the pMT1 ParA and ParB proteins were supplied in trans , showing that the pMT1 par system is fully functional for plasmid partition in E. coli . The pMT1 par system exerted a plasmid silencing activity similar to, but weaker than those of P7 par and P1 par . In spite of the high degree of similarity, especially to P7 par , it showed unique specificities with respect to the interactions of key components. Neither the P7 nor P1 Par proteins could support partition via the pMT1 parS site, and the pMT1 Par proteins failed to support partition with P1 parS or P7 parS . Typical of other partition sites, supernumerary copies of pMT1 parS exerted incompatibility toward plasmids supported by pMT1 par . However, no interspecies incompatibility effect was observed between pMT1par, P7 par , and P1 par ." @default.
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- W2075606418 date "2000-07-15" @default.
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- W2075606418 title "A Plasmid Partition System of the P1-P7 par Family from the pMT1 Virulence Plasmid of Yersinia pestis" @default.
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