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- W2075608223 abstract "A distinct poly(A) − RNA sedimenting around 10–12S was identified during spore germination in Dictyostelium discoideum. Activated spores were labeled with [ 3 H]uracil and the poly(A) − RNA was purified from ribosomal particles for analysis. In the spore swelling stage, 40 to 50% of the newly synthesized poly(A) − RNA was 10–12S RNA. This fraction diminished to one-half or one-fourth depending on the labeling period at the stage of amoeba emergence. The 10–12S RNA was associated with both monosomes and polysomes in vivo. Translation in a wheat germ cell-free system and gel electrophoresis demonstrated that the 10–12S RNA coded for a number of polypeptides, some of which were also represented among the in vitro products of poly(A) + RNA. However, there were seven unique polypeptides (37.5, 28.2, 27.5, 23, 17.7, 17, and 14.2 kilodaltons) encoded exclusively by 10–12S RNA.Key words: cellular slime mold, RNA synthesis, development, poly(A) − mRNA, in vitro translation." @default.
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- W2075608223 date "1989-05-01" @default.
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- W2075608223 title "Synthesis of a ribosome-bound translatable poly(A)− mRNA during spore germination in Dictyostelium discoideum" @default.
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- W2075608223 doi "https://doi.org/10.1139/m89-091" @default.
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