FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2075693670> ?p ?o ?g. }

• W2075693670 endingPage "117" @default.
• W2075693670 startingPage "107" @default.
• W2075693670 abstract "Ca2+-sensitive adenylyl cyclases (ACs) orchestrate dynamic interplay between Ca2+ and cAMP that is a crucial feature of cellular homeostasis. Significantly, these ACs are highly selective for capacitative Ca2+ entry (CCE) over other modes of Ca2+ increase. To directly address the possibility that these ACs reside in discrete Ca2+ microdomains, we tethered a Ca2+ sensor, GCaMP2, to the N-terminus of Ca2+-stimulated AC8. GCaMP2-AC8 measurements were compared with global, plasma membrane (PM)-targeted or Ca2+-insensitive AC2-targeted GCaMP2. In intact cells, GCaMP2-AC8 responded rapidly to CCE, but was largely unresponsive to other types of Ca2+ rise. The global GCaMP2, PM-targeted GCaMP2 and GCaMP2-AC2 sensors reported large Ca2+ fluxes during Ca2+ mobilization and non-specific Ca2+ entry, but were less responsive to CCE than GCaMP2-AC8. Our data reveal that different AC isoforms localize to distinct Ca2+-microdomains within the plasma membrane. AC2, which is regulated via protein kinase C, resides in a microdomain that is exposed to a range of widespread Ca2+ signals seen throughout the cytosol. By contrast, a unique Ca2+ microdomain surrounds AC8 that promotes selectivity for Ca2+ signals arising from CCE, and optimizes CCE-mediated cAMP synthesis. This direct demonstration of discrete compartmentalized Ca2+ signals associated with specific signalling proteins provides a remarkable insight into the functional organization of signalling microdomains." @default.
• W2075693670 created "2016-06-24" @default.
• W2075693670 creator A5003176081 @default.
• W2075693670 creator A5064524946 @default.
• W2075693670 creator A5082828225 @default.
• W2075693670 creator A5087794452 @default.
• W2075693670 date "2010-01-01" @default.
• W2075693670 modified "2023-09-27" @default.
• W2075693670 title "Direct demonstration of discrete Ca2+ microdomains associated with different isoforms of adenylyl cyclase" @default.
• W2075693670 cites W1490301028 @default.
• W2075693670 cites W1521077143 @default.
• W2075693670 cites W1567653349 @default.
• W2075693670 cites W1571164133 @default.
• W2075693670 cites W1590540783 @default.
• W2075693670 cites W1606703336 @default.
• W2075693670 cites W1670760110 @default.
• W2075693670 cites W1969387286 @default.
• W2075693670 cites W1970353708 @default.
• W2075693670 cites W1970468568 @default.
• W2075693670 cites W1971748110 @default.
• W2075693670 cites W1975042562 @default.
• W2075693670 cites W1990499518 @default.
• W2075693670 cites W1995536774 @default.
• W2075693670 cites W2000121694 @default.
• W2075693670 cites W2008374286 @default.
• W2075693670 cites W2010694064 @default.
• W2075693670 cites W2011921884 @default.
• W2075693670 cites W2012057989 @default.
• W2075693670 cites W2014866096 @default.
• W2075693670 cites W2016701637 @default.
• W2075693670 cites W2023556678 @default.
• W2075693670 cites W2026264437 @default.
• W2075693670 cites W2036513858 @default.
• W2075693670 cites W2039923059 @default.
• W2075693670 cites W2055713680 @default.
• W2075693670 cites W2058449890 @default.
• W2075693670 cites W2061194293 @default.
• W2075693670 cites W2072484997 @default.
• W2075693670 cites W2072515967 @default.
• W2075693670 cites W2075389941 @default.
• W2075693670 cites W2078817376 @default.
• W2075693670 cites W2079448952 @default.
• W2075693670 cites W2079499409 @default.
• W2075693670 cites W2083049815 @default.
• W2075693670 cites W2085275630 @default.
• W2075693670 cites W2087925323 @default.
• W2075693670 cites W2090007562 @default.
• W2075693670 cites W2090363261 @default.
• W2075693670 cites W2091278154 @default.
• W2075693670 cites W2105563311 @default.
• W2075693670 cites W2105951582 @default.
• W2075693670 cites W2113943014 @default.
• W2075693670 cites W2116999551 @default.
• W2075693670 cites W2127166394 @default.
• W2075693670 cites W2133603157 @default.
• W2075693670 cites W2133844804 @default.
• W2075693670 cites W2136460746 @default.
• W2075693670 cites W2137137793 @default.
• W2075693670 cites W2151519853 @default.
• W2075693670 cites W2156132267 @default.
• W2075693670 cites W2170627923 @default.
• W2075693670 cites W2331390021 @default.
• W2075693670 cites W2409628269 @default.
• W2075693670 doi "https://doi.org/10.1242/jcs.062067" @default.
• W2075693670 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2794712" @default.
• W2075693670 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20016071" @default.
• W2075693670 hasPublicationYear "2010" @default.
• W2075693670 type Work @default.
• W2075693670 sameAs 2075693670 @default.
• W2075693670 citedByCount "53" @default.
• W2075693670 countsByYear W20756936702012 @default.
• W2075693670 countsByYear W20756936702013 @default.
• W2075693670 countsByYear W20756936702014 @default.
• W2075693670 countsByYear W20756936702015 @default.
• W2075693670 countsByYear W20756936702016 @default.
• W2075693670 countsByYear W20756936702017 @default.
• W2075693670 countsByYear W20756936702018 @default.
• W2075693670 countsByYear W20756936702019 @default.
• W2075693670 countsByYear W20756936702021 @default.
• W2075693670 crossrefType "journal-article" @default.
• W2075693670 hasAuthorship W2075693670A5003176081 @default.
• W2075693670 hasAuthorship W2075693670A5064524946 @default.
• W2075693670 hasAuthorship W2075693670A5082828225 @default.
• W2075693670 hasAuthorship W2075693670A5087794452 @default.
• W2075693670 hasBestOaLocation W20756936701 @default.
• W2075693670 hasConcept C104317684 @default.
• W2075693670 hasConcept C134088382 @default.
• W2075693670 hasConcept C181199279 @default.
• W2075693670 hasConcept C201571599 @default.
• W2075693670 hasConcept C2779178603 @default.
• W2075693670 hasConcept C41625074 @default.
• W2075693670 hasConcept C53345823 @default.
• W2075693670 hasConcept C55493867 @default.
• W2075693670 hasConcept C62478195 @default.
• W2075693670 hasConcept C63645605 @default.
• W2075693670 hasConcept C86803240 @default.
• W2075693670 hasConcept C95444343 @default.
• W2075693670 hasConcept C98539663 @default.

• next