FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2075732157> ?p ?o ?g. }

• W2075732157 endingPage "2582" @default.
• W2075732157 startingPage "2561" @default.
• W2075732157 abstract "The present work shows the characterization of Phaseolus acutifolius variety latifolius, on which little research has been published, and provides detailed information on the corresponding lectin. This protein was purified from a semi-domesticated line of white tepary beans from Sonora, Mexico, by precipitation of the aqueous extract with ammonium sulfate, followed by affinity chromatography on an immobilized fetuin matrix. MALDI TOF analysis of Phaseolus acutifolius agglutinin (PAA) showed that this lectin is composed of monomers with molecular weights ranging between 28 and 31 kDa. At high salt concentrations, PAA forms a dimer of 63 kDa, but at low salt concentrations, the subunits form a tetramer. Analysis of PAA on 2D-PAGE showed that there are mainly three types of subunits with isoelectric points of 4.2, 4.4, and 4.5. The partial sequence obtained by LC/MS/MS of tryptic fragments from the PAA subunits showed 90–100% identity with subunits from genus Phaseolus lectins in previous reports. The tepary bean lectin showed lower hemagglutination activity than Phaseolus vulgaris hemagglutinin (PHA-E) toward trypsinized human A and O type erythrocytes. The hemagglutination activity was inhibited by N-glycans from glycoproteins. Affinity chromatography with the immobilized PAA showed a high affinity to glycopeptides from thyroglobulin, which also has N-glycans with a high content of N-acetylglucosamine. PAA showed less mitogenic activity toward human lymphocytes than PHA-L and Con A. The cytotoxicity of PAA was determined by employing three clones of the 3T3 cell line, demonstrating variability among the clones as follows: T4 (DI50 51.5 µg/mL); J20 (DI50 275 µg/mL), and N5 (DI50 72.5 µg/mL)." @default.
• W2075732157 created "2016-06-24" @default.
• W2075732157 creator A5012162895 @default.
• W2075732157 creator A5025644783 @default.
• W2075732157 creator A5029684072 @default.
• W2075732157 creator A5030455925 @default.
• W2075732157 creator A5043071507 @default.
• W2075732157 creator A5065367333 @default.
• W2075732157 creator A5069885221 @default.
• W2075732157 creator A5083464566 @default.
• W2075732157 creator A5088411423 @default.
• W2075732157 creator A5091525533 @default.
• W2075732157 date "2011-03-21" @default.
• W2075732157 modified "2023-09-27" @default.
• W2075732157 title "Purification, Biochemical Characterization, and Bioactive Properties of a Lectin Purified from the Seeds of White Tepary Bean (Phaseolus Acutifolius Variety Latifolius)" @default.
• W2075732157 cites W1485602773 @default.
• W2075732157 cites W1511644631 @default.
• W2075732157 cites W1517161569 @default.
• W2075732157 cites W1540450900 @default.
• W2075732157 cites W1542816686 @default.
• W2075732157 cites W1563704931 @default.
• W2075732157 cites W1567114658 @default.
• W2075732157 cites W1577360674 @default.
• W2075732157 cites W1578874979 @default.
• W2075732157 cites W1594583996 @default.
• W2075732157 cites W1661742214 @default.
• W2075732157 cites W1775749144 @default.
• W2075732157 cites W1821925018 @default.
• W2075732157 cites W1968834637 @default.
• W2075732157 cites W1971269557 @default.
• W2075732157 cites W1973358741 @default.
• W2075732157 cites W1977910515 @default.
• W2075732157 cites W1978021382 @default.
• W2075732157 cites W1979492674 @default.
• W2075732157 cites W1986699235 @default.
• W2075732157 cites W1996266454 @default.
• W2075732157 cites W1996878758 @default.
• W2075732157 cites W1997557737 @default.
• W2075732157 cites W2001793813 @default.
• W2075732157 cites W2002613342 @default.
• W2075732157 cites W2009647705 @default.
• W2075732157 cites W2010850086 @default.
• W2075732157 cites W2012458218 @default.
• W2075732157 cites W2014076781 @default.
• W2075732157 cites W2014307378 @default.
• W2075732157 cites W2027129623 @default.
• W2075732157 cites W2031379110 @default.
• W2075732157 cites W2051418839 @default.
• W2075732157 cites W2053293806 @default.
• W2075732157 cites W2053320706 @default.
• W2075732157 cites W2053985816 @default.
• W2075732157 cites W2054526986 @default.
• W2075732157 cites W2058846315 @default.
• W2075732157 cites W2060659016 @default.
• W2075732157 cites W2061596803 @default.
• W2075732157 cites W2067067189 @default.
• W2075732157 cites W2069573754 @default.
• W2075732157 cites W2073056717 @default.
• W2075732157 cites W2073253049 @default.
• W2075732157 cites W2073941162 @default.
• W2075732157 cites W2086048344 @default.
• W2075732157 cites W2086704971 @default.
• W2075732157 cites W2087714682 @default.
• W2075732157 cites W2097697129 @default.
• W2075732157 cites W2098442702 @default.
• W2075732157 cites W2100837269 @default.
• W2075732157 cites W2107595196 @default.
• W2075732157 cites W2120618486 @default.
• W2075732157 cites W2121143789 @default.
• W2075732157 cites W2127646013 @default.
• W2075732157 cites W2152161949 @default.
• W2075732157 cites W2171677744 @default.
• W2075732157 cites W2345552382 @default.
• W2075732157 cites W2515603268 @default.
• W2075732157 doi "https://doi.org/10.3390/molecules16032561" @default.
• W2075732157 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6259754" @default.
• W2075732157 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21441861" @default.
• W2075732157 hasPublicationYear "2011" @default.
• W2075732157 type Work @default.
• W2075732157 sameAs 2075732157 @default.
• W2075732157 citedByCount "22" @default.
• W2075732157 countsByYear W20757321572012 @default.
• W2075732157 countsByYear W20757321572013 @default.
• W2075732157 countsByYear W20757321572014 @default.
• W2075732157 countsByYear W20757321572015 @default.
• W2075732157 countsByYear W20757321572017 @default.
• W2075732157 countsByYear W20757321572018 @default.
• W2075732157 countsByYear W20757321572019 @default.
• W2075732157 countsByYear W20757321572020 @default.
• W2075732157 countsByYear W20757321572021 @default.
• W2075732157 countsByYear W20757321572022 @default.
• W2075732157 crossrefType "journal-article" @default.
• W2075732157 hasAuthorship W2075732157A5012162895 @default.
• W2075732157 hasAuthorship W2075732157A5025644783 @default.
• W2075732157 hasAuthorship W2075732157A5029684072 @default.
• W2075732157 hasAuthorship W2075732157A5030455925 @default.
• W2075732157 hasAuthorship W2075732157A5043071507 @default.
• W2075732157 hasAuthorship W2075732157A5065367333 @default.

• next