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• W2075739543 abstract "A newly devised procedure for a simultaneous determination of urinary tetrahydroaldosterone and aldosterone is described. The procedure is based on deconjugation and acetalization, followed by extraction and derivatization of the urinary compounds to their trimethylsilyl ethers and subsequent gas chromatographic-mass fragmentographic detection. To evaluate the assay, aliquots of a urine sample of a healthy individual were analysed in multiplicate; a mean tetrahydroaidosterone concentration of 103 nmol/l and a within sample, within-day- and day-to-day coefficient of variation of 1.8, 3.2 and 3.4%, respectively, were found. Determination of aldosterone in the same sample yielded a mean concentration of 25.3 nmol/l and the following coefficients of variation: 2.8% (within-sample), 3.8% (within-day) and 4.3% (day-to-day). The urinary excretion of tetrahydroaldosterone and aldosterone in 24-h urine portions was determined in twenty healthy individuals, aged 23–77 years; for tetrahydroaldosterone and aldosterone, an excretion of 94 ± 66 nmol per 24 h and of 40 ± 22 nmol per 24 h was found, respectively, in accord with the literature. An example of the usefulness of the described assay is given by establishing the cause of severe salt-wasting in an infant; a highly elevated tetrahydroaldosterone and aldosterone excretion was demonstrated, proving that the child suffered from unresponsiveness to aldosterone (pseudohypoaldosteronism)." @default.
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• W2075739543 date "1986-01-01" @default.
• W2075739543 modified "2023-09-26" @default.
• W2075739543 title "Improved gas chromatographic—mass fragmentographic assay for tetrahydroaldosterone and aldosterone in urine" @default.
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• W2075739543 doi "https://doi.org/10.1016/s0378-4347(00)80725-2" @default.
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