Matches in SemOpenAlex for { <https://semopenalex.org/work/W2075838863> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2075838863 abstract "It is important to distinguish between Bacteroides fragilis strains as their virulence may vary and as B. fragilis seems to be a heterogeneous species. The aim of our study was to evaluate ribotyping for differentiation of 46 strains of B. fragilis and for assessment of strain heterogeneity within and between the two DNA-DNA homology strain groups established in this species. Twenty-seven strains belonged to Johnson's DNA homology group I and eight to group II. Eleven strains had not been assigned to any group (NI group). DNA from all strains was cut with BglI, EcoRI and HindIII. Restriction fragment length polymorphisms were investigated using a non-radioactive digoxigenin-labelled cDNA probe transcribed from Escherichia coli 16S+23S rRNA. Ribotyping with BglI was most discriminatory, revealing a total of 26 different patterns by visual inspection of gels. EcoRI followed with 20 patterns and HindIII with 13 patterns. The gels from ribotyping were processed using the Dendron computer-assisted program. Strain clusters established using Dendron were not always in agreement with homology-based strain groups. Strains of the NI group fell into both homology groups. Ribotyping, as it is based on a relatively small portion of the genome, is useful for strain distinction in epidemiological studies with B. fragilis, whereas DNA-DNA homology, using the entire genome, is more reliable for taxonomy. The Dendron computer-assisted program, which enabled objective assessment of multiple banding patterns, increased the reliability of ribotyping." @default.
- W2075838863 created "2016-06-24" @default.
- W2075838863 creator A5056060600 @default.
- W2075838863 creator A5063541662 @default.
- W2075838863 date "2000-06-01" @default.
- W2075838863 modified "2023-09-24" @default.
- W2075838863 title "Strain differentiation in Bacteroides fragilis by ribotyping and computer-assisted gel analysisNote" @default.
- W2075838863 doi "https://doi.org/10.1034/j.1600-0463.2000.d01-79.x" @default.
- W2075838863 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11028806" @default.
- W2075838863 hasPublicationYear "2000" @default.
- W2075838863 type Work @default.
- W2075838863 sameAs 2075838863 @default.
- W2075838863 citedByCount "6" @default.
- W2075838863 countsByYear W20758388632013 @default.
- W2075838863 crossrefType "journal-article" @default.
- W2075838863 hasAuthorship W2075838863A5056060600 @default.
- W2075838863 hasAuthorship W2075838863A5063541662 @default.
- W2075838863 hasConcept C104317684 @default.
- W2075838863 hasConcept C128319531 @default.
- W2075838863 hasConcept C130123407 @default.
- W2075838863 hasConcept C151292725 @default.
- W2075838863 hasConcept C153911025 @default.
- W2075838863 hasConcept C165525559 @default.
- W2075838863 hasConcept C2777368272 @default.
- W2075838863 hasConcept C2780246119 @default.
- W2075838863 hasConcept C49105822 @default.
- W2075838863 hasConcept C523546767 @default.
- W2075838863 hasConcept C54355233 @default.
- W2075838863 hasConcept C552990157 @default.
- W2075838863 hasConcept C86803240 @default.
- W2075838863 hasConcept C89423630 @default.
- W2075838863 hasConceptScore W2075838863C104317684 @default.
- W2075838863 hasConceptScore W2075838863C128319531 @default.
- W2075838863 hasConceptScore W2075838863C130123407 @default.
- W2075838863 hasConceptScore W2075838863C151292725 @default.
- W2075838863 hasConceptScore W2075838863C153911025 @default.
- W2075838863 hasConceptScore W2075838863C165525559 @default.
- W2075838863 hasConceptScore W2075838863C2777368272 @default.
- W2075838863 hasConceptScore W2075838863C2780246119 @default.
- W2075838863 hasConceptScore W2075838863C49105822 @default.
- W2075838863 hasConceptScore W2075838863C523546767 @default.
- W2075838863 hasConceptScore W2075838863C54355233 @default.
- W2075838863 hasConceptScore W2075838863C552990157 @default.
- W2075838863 hasConceptScore W2075838863C86803240 @default.
- W2075838863 hasConceptScore W2075838863C89423630 @default.
- W2075838863 hasLocation W20758388631 @default.
- W2075838863 hasLocation W20758388632 @default.
- W2075838863 hasOpenAccess W2075838863 @default.
- W2075838863 hasPrimaryLocation W20758388631 @default.
- W2075838863 hasRelatedWork W1623795590 @default.
- W2075838863 hasRelatedWork W1802929309 @default.
- W2075838863 hasRelatedWork W1968535898 @default.
- W2075838863 hasRelatedWork W1982728459 @default.
- W2075838863 hasRelatedWork W1986573094 @default.
- W2075838863 hasRelatedWork W2002390941 @default.
- W2075838863 hasRelatedWork W2004188260 @default.
- W2075838863 hasRelatedWork W2019891895 @default.
- W2075838863 hasRelatedWork W2024272159 @default.
- W2075838863 hasRelatedWork W2037830338 @default.
- W2075838863 hasRelatedWork W2053080974 @default.
- W2075838863 hasRelatedWork W2062336252 @default.
- W2075838863 hasRelatedWork W2066474493 @default.
- W2075838863 hasRelatedWork W2080007168 @default.
- W2075838863 hasRelatedWork W2083958534 @default.
- W2075838863 hasRelatedWork W2092593512 @default.
- W2075838863 hasRelatedWork W2124874582 @default.
- W2075838863 hasRelatedWork W2146509092 @default.
- W2075838863 hasRelatedWork W2164583245 @default.
- W2075838863 hasRelatedWork W2165133016 @default.
- W2075838863 isParatext "false" @default.
- W2075838863 isRetracted "false" @default.
- W2075838863 magId "2075838863" @default.
- W2075838863 workType "article" @default.