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• W2075843949 abstract "Metallic nanoparticles (MNPs) are known to alter the emission of vicinal fluorophores through the near-field interaction, leading to either fluorescence quenching or enhancement. Much ambiguity remains in the experimental outcome of such a near-field interaction, particularly for bulk colloidal solution. It is hypothesized that the strong far-field interference from the inner filter effect of the MNPs could mask the true near-field MNP–fluorophore interaction significantly. Thus, in this work, a reliable internal control capable of decoupling the near-field interaction from far-field interference is established by the use of the DNA toehold concept to mediate the in situ assembly and disassembly of the MNP–fluorophore conjugate. A model gold nanoparticle (AuNP)–Cy3 system is used to investigate our proposed toehold-mediated internal control system. The maximum fluorescence enhancement is obtained for large-sized AuNP (58 nm) separated from Cy3 at an intermediate distance of 6.8 nm, while fluorescence quenching is observed for smaller-sized AuNP (11 nm and 23 nm), which is in agreement with the theoretical values reported in the literature. This work shows that the toehold-mediated internal control design can serve as a central system for evaluating the near-field interaction of other MNP–fluorophore combinations and facilitate the rational design of specific MNP–fluorophore systems for various applications." @default.
• W2075843949 created "2016-06-24" @default.
• W2075843949 creator A5033188108 @default.
• W2075843949 creator A5070488490 @default.
• W2075843949 date "2014-09-02" @default.
• W2075843949 modified "2023-10-02" @default.
• W2075843949 title "Toehold-mediated internal control to probe the near-field interaction between the metallic nanoparticle and the fluorophore" @default.
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• W2075843949 doi "https://doi.org/10.1039/c4nr03643c" @default.
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