FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2075905536> ?p ?o ?g. }

• W2075905536 endingPage "30" @default.
• W2075905536 startingPage "21" @default.
• W2075905536 abstract "The present experiment used cultured mouse cumulus cell-enclosed oocytes (CEOs) and denuded oocytes (DOs) to study the function of nitric oxide (NO) in mouse oocyte meiotic maturation. Either positive or negative actions of NO on meiotic maturation has been observed when CEOs or DOs were cultured for 24 h in a medium containing 4 mM hypoxanthine (HX) to maintain meiotic arrest, or in maturation medium (without HX) supplemented with different doses of sodium nitroprusside (SNP, a NO donor), N-omega-nitro-L-arginine methyl ester (L-NAME) or N(w)-nitro-L-arginine (L-NNA) (two inhibitors of NO synthase, NOS), and L-arginine (the only substrate of NOS). Both NOS inhibitors suppressed the formation of first polar body (PB1) of the oocytes in CEOs in a dose dependent manner, but no effect on germinal vesicle break down (GVBD) was observed. An optimal inhibitory effect was observed with either 10(-3) M L-NAME (P<0.01) or 10(-3) M L-NNA (P<0.01) and the inhibition could be reversed by the addition of SNP (10(-5) M). The above mentioned optimal concentration of L-NAME or L-NNA on CEOs exhibited no effect on oocyte meiotic maturation of DOs. Treatments of low concentrations of SNP (10(-7), 10(-6), 10(-5) M) stimulated significantly the oocyte meiotic maturation of CEOs which were inhibited with HX, but had no effect on DOs in the same culture medium. While, the treatment with high concentrations of SNP (0.1-4 mM) during the CEOs cultured in maturation medium resulted in a lower percentage of oocytes at PB1 stage and a higher percentage of atypical oocytes in a dose dependent manner compared with control. A dose of SNP at 1 mM exhibited significant inhibitory effect on the formation of PB1, but without effect on the number of atypical oocytes compared with control, while, this SNP dosage not only inhibited the oocyte PB1 formation but also increased the percentage of dead oocytes in DOs. Although oocytes of all groups underwent GVBD at the end of the culture in the spontaneous maturation medium, the results of the kinetics showed that the treatment of the optimal concentration of SNP (1 mM) could significantly delay GVBD during the first 5 h culture period. The concomitant addition of L-NAME with SNP did not reverse the inhibitory effect of SNP on CEOs. Similarly, neither pre-incubation nor illumination by ultraviolet ray could balance the inhibitory effect of SNP. Finally, when added alone at a concentration of 4 mM, L-arg caused extensive death of both CEOs and DOs. While, administration of 4 mM L-arg and 1 mM L-NAME to both CEOs and DOs simultaneously resulted in markedly reduced CEOs death percentage as compared with L-arg treatment alone, but not in DOs. These data support the idea that NO could act with a dual action (stimulation or inhibition) in mouse meiotic maturation depending on its concentration." @default.
• W2075905536 created "2016-06-24" @default.
• W2075905536 creator A5022261541 @default.
• W2075905536 creator A5025006462 @default.
• W2075905536 creator A5039360390 @default.
• W2075905536 creator A5069415097 @default.
• W2075905536 creator A5085325774 @default.
• W2075905536 date "2003-09-01" @default.
• W2075905536 modified "2023-10-16" @default.
• W2075905536 title "Dual effects of nitric oxide on meiotic maturation of mouse cumulus cell-enclosed oocytes in vitro" @default.
• W2075905536 cites W1821918060 @default.
• W2075905536 cites W1978468242 @default.
• W2075905536 cites W1978570830 @default.
• W2075905536 cites W1983392326 @default.
• W2075905536 cites W1986140299 @default.
• W2075905536 cites W1986875908 @default.
• W2075905536 cites W1992741767 @default.
• W2075905536 cites W1999376129 @default.
• W2075905536 cites W2009013636 @default.
• W2075905536 cites W2009760139 @default.
• W2075905536 cites W2010844653 @default.
• W2075905536 cites W2011348946 @default.
• W2075905536 cites W2017217180 @default.
• W2075905536 cites W2020534176 @default.
• W2075905536 cites W2022760639 @default.
• W2075905536 cites W2023170094 @default.
• W2075905536 cites W2033379487 @default.
• W2075905536 cites W2034282657 @default.
• W2075905536 cites W2044918962 @default.
• W2075905536 cites W2048899274 @default.
• W2075905536 cites W2049693640 @default.
• W2075905536 cites W2053299519 @default.
• W2075905536 cites W2054167608 @default.
• W2075905536 cites W2082237694 @default.
• W2075905536 cites W2091027017 @default.
• W2075905536 cites W2096505980 @default.
• W2075905536 cites W2102094264 @default.
• W2075905536 cites W2107732949 @default.
• W2075905536 cites W2108780176 @default.
• W2075905536 cites W2130539014 @default.
• W2075905536 cites W2134953226 @default.
• W2075905536 cites W2139701619 @default.
• W2075905536 cites W2151355223 @default.
• W2075905536 cites W2169685951 @default.
• W2075905536 cites W2170530908 @default.
• W2075905536 cites W2395453807 @default.
• W2075905536 cites W4211135707 @default.
• W2075905536 cites W4230158867 @default.
• W2075905536 cites W4232855929 @default.
• W2075905536 cites W4233450424 @default.
• W2075905536 cites W4234846948 @default.
• W2075905536 cites W4238948349 @default.
• W2075905536 cites W4247162454 @default.
• W2075905536 doi "https://doi.org/10.1016/s0303-7207(03)00213-2" @default.
• W2075905536 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12972180" @default.
• W2075905536 hasPublicationYear "2003" @default.
• W2075905536 type Work @default.
• W2075905536 sameAs 2075905536 @default.
• W2075905536 citedByCount "85" @default.
• W2075905536 countsByYear W20759055362012 @default.
• W2075905536 countsByYear W20759055362013 @default.
• W2075905536 countsByYear W20759055362014 @default.
• W2075905536 countsByYear W20759055362015 @default.
• W2075905536 countsByYear W20759055362016 @default.
• W2075905536 countsByYear W20759055362017 @default.
• W2075905536 countsByYear W20759055362018 @default.
• W2075905536 countsByYear W20759055362019 @default.
• W2075905536 countsByYear W20759055362020 @default.
• W2075905536 countsByYear W20759055362021 @default.
• W2075905536 countsByYear W20759055362022 @default.
• W2075905536 crossrefType "journal-article" @default.
• W2075905536 hasAuthorship W2075905536A5022261541 @default.
• W2075905536 hasAuthorship W2075905536A5025006462 @default.
• W2075905536 hasAuthorship W2075905536A5039360390 @default.
• W2075905536 hasAuthorship W2075905536A5069415097 @default.
• W2075905536 hasAuthorship W2075905536A5085325774 @default.
• W2075905536 hasConcept C104317684 @default.
• W2075905536 hasConcept C134018914 @default.
• W2075905536 hasConcept C148196450 @default.
• W2075905536 hasConcept C16685009 @default.
• W2075905536 hasConcept C185592680 @default.
• W2075905536 hasConcept C196843134 @default.
• W2075905536 hasConcept C2775966511 @default.
• W2075905536 hasConcept C2776690073 @default.
• W2075905536 hasConcept C2777468819 @default.
• W2075905536 hasConcept C2777622882 @default.
• W2075905536 hasConcept C2779610285 @default.
• W2075905536 hasConcept C2994119143 @default.
• W2075905536 hasConcept C515207424 @default.
• W2075905536 hasConcept C519581460 @default.
• W2075905536 hasConcept C55493867 @default.
• W2075905536 hasConcept C71924100 @default.
• W2075905536 hasConcept C86803240 @default.
• W2075905536 hasConcept C95444343 @default.
• W2075905536 hasConceptScore W2075905536C104317684 @default.
• W2075905536 hasConceptScore W2075905536C134018914 @default.
• W2075905536 hasConceptScore W2075905536C148196450 @default.
• W2075905536 hasConceptScore W2075905536C16685009 @default.

• next