Matches in SemOpenAlex for { <https://semopenalex.org/work/W2075933012> ?p ?o ?g. }
- W2075933012 endingPage "625" @default.
- W2075933012 startingPage "618" @default.
- W2075933012 abstract "Epitope tags have been increasingly used to understand ion channel subunit assembly and interaction, trafficking, subcellular localization, and function in living cells. In particular, epitope tags have proven extremely useful for analyses of closely related, highly homologous channel subunits in endogenous cell contexts in vitro and in vivo, where multiple channel isoforms may be expressed. However, as the variety of epitope tags that have been used has expanded, and the use of tagged channel subunits has become increasingly sophisticated and widespread, there has also been an increase in the number of examples highlighting the potential problems associated with the use of epitope tags for ion channel studies. Described here are some of the epitope tags that have been used to study ion channel subunits, including the HA, FLAG, myc, His6, and green fluorescent protein (GFP) epitopes, as well as some of the applications and avenues of research in which they have proven advantageous. Potential pitfalls and caveats associated with the use of these epitope tags are also discussed, with an emphasis on the need to include careful characterization of epitope-tagged channel subunits as part of their construction. Finally, potential avenues for future investigation and the development of this approach are considered." @default.
- W2075933012 created "2016-06-24" @default.
- W2075933012 creator A5083630596 @default.
- W2075933012 date "2007-09-11" @default.
- W2075933012 modified "2023-10-10" @default.
- W2075933012 title "Understanding ion channel biology using epitope tags: Progress, pitfalls, and promise" @default.
- W2075933012 cites W1515496828 @default.
- W2075933012 cites W1534736058 @default.
- W2075933012 cites W1537007267 @default.
- W2075933012 cites W1564531279 @default.
- W2075933012 cites W1594010048 @default.
- W2075933012 cites W1629056281 @default.
- W2075933012 cites W1664511950 @default.
- W2075933012 cites W1826524642 @default.
- W2075933012 cites W1930335657 @default.
- W2075933012 cites W1934415635 @default.
- W2075933012 cites W1937176890 @default.
- W2075933012 cites W1967187871 @default.
- W2075933012 cites W1969133615 @default.
- W2075933012 cites W1969253956 @default.
- W2075933012 cites W1971479881 @default.
- W2075933012 cites W1971826341 @default.
- W2075933012 cites W1975353055 @default.
- W2075933012 cites W1980360147 @default.
- W2075933012 cites W1982236694 @default.
- W2075933012 cites W1986804563 @default.
- W2075933012 cites W1987785656 @default.
- W2075933012 cites W1988051872 @default.
- W2075933012 cites W1988326309 @default.
- W2075933012 cites W1989688231 @default.
- W2075933012 cites W1992660876 @default.
- W2075933012 cites W1994304837 @default.
- W2075933012 cites W1995364413 @default.
- W2075933012 cites W1997662437 @default.
- W2075933012 cites W1997808465 @default.
- W2075933012 cites W1998079894 @default.
- W2075933012 cites W2004313744 @default.
- W2075933012 cites W2006168822 @default.
- W2075933012 cites W2008267845 @default.
- W2075933012 cites W2008373413 @default.
- W2075933012 cites W2008560382 @default.
- W2075933012 cites W2012430176 @default.
- W2075933012 cites W2012785062 @default.
- W2075933012 cites W2012940954 @default.
- W2075933012 cites W2013412105 @default.
- W2075933012 cites W2013575118 @default.
- W2075933012 cites W2014293907 @default.
- W2075933012 cites W2016367251 @default.
- W2075933012 cites W2017924946 @default.
- W2075933012 cites W2021825901 @default.
- W2075933012 cites W2021896725 @default.
- W2075933012 cites W2027479454 @default.
- W2075933012 cites W2028179204 @default.
- W2075933012 cites W2029031422 @default.
- W2075933012 cites W2030695915 @default.
- W2075933012 cites W2030814128 @default.
- W2075933012 cites W2032250137 @default.
- W2075933012 cites W2032339475 @default.
- W2075933012 cites W2032710773 @default.
- W2075933012 cites W2032965800 @default.
- W2075933012 cites W2033129372 @default.
- W2075933012 cites W2034728504 @default.
- W2075933012 cites W2039538170 @default.
- W2075933012 cites W2039692671 @default.
- W2075933012 cites W2041119907 @default.
- W2075933012 cites W2042463836 @default.
- W2075933012 cites W2042845431 @default.
- W2075933012 cites W2043055409 @default.
- W2075933012 cites W2043351896 @default.
- W2075933012 cites W2044679188 @default.
- W2075933012 cites W2045006795 @default.
- W2075933012 cites W2045626047 @default.
- W2075933012 cites W2046662604 @default.
- W2075933012 cites W2046722466 @default.
- W2075933012 cites W2047559848 @default.
- W2075933012 cites W2047598222 @default.
- W2075933012 cites W2053142643 @default.
- W2075933012 cites W2057635423 @default.
- W2075933012 cites W2059911949 @default.
- W2075933012 cites W2060229932 @default.
- W2075933012 cites W2060426324 @default.
- W2075933012 cites W2060552473 @default.
- W2075933012 cites W2061046931 @default.
- W2075933012 cites W2061177065 @default.
- W2075933012 cites W2063807479 @default.
- W2075933012 cites W2066246595 @default.
- W2075933012 cites W2072449934 @default.
- W2075933012 cites W2075450053 @default.
- W2075933012 cites W2075816147 @default.
- W2075933012 cites W2078155821 @default.
- W2075933012 cites W2084778806 @default.
- W2075933012 cites W2085045611 @default.
- W2075933012 cites W2085955152 @default.
- W2075933012 cites W2086847004 @default.
- W2075933012 cites W2092759474 @default.
- W2075933012 cites W2094212507 @default.
- W2075933012 cites W2095930061 @default.
- W2075933012 cites W2097678345 @default.