FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2075970918> ?p ?o ?g. }

• W2075970918 endingPage "432" @default.
• W2075970918 startingPage "419" @default.
• W2075970918 abstract "A mechanism for the precise excision of DNA between two target sites was elucidated by analysing the individual steps during the reactions of the SfiI endonuclease on a plasmid with two SfiI sites. Previous studies had indicated that SfiI is a tetrameric protein that binds to two copies of its recognition site before cleaving both sites in both strands. In this study, the concerted cleavage of four phosphodiester bonds was shown to arise from four consecutive reactions that had similar values for their intrinsic rate constants. Each reaction is presumably mediated by one of the four active sites in the tetramer and all four were generally completed within the life-time of the complex between the protein and two recognition sites, though products cleaved in one or two phosphodiester bonds were also detected following premature dissociation of the enzyme-substrate complex at elevated temperatures. At the physiological temperature for this enzyme, all four bonds were cleaved within one minute but the subsequent dissociation of the enzyme-product complex, liberating the excised segment of DNA, took about one hour. The tetrameric structure for SfiI was confirmed by equilibrium centrifugation." @default.
• W2075970918 created "2016-06-24" @default.
• W2075970918 creator A5011374485 @default.
• W2075970918 creator A5021651233 @default.
• W2075970918 creator A5069818913 @default.
• W2075970918 creator A5087300006 @default.
• W2075970918 date "1998-08-01" @default.
• W2075970918 modified "2023-10-04" @default.
• W2075970918 title "DNA excision by the SfiI restriction endonuclease" @default.
• W2075970918 cites W134540825 @default.
• W2075970918 cites W1520249041 @default.
• W2075970918 cites W1538904365 @default.
• W2075970918 cites W1590546489 @default.
• W2075970918 cites W1604559018 @default.
• W2075970918 cites W1972072721 @default.
• W2075970918 cites W1972824210 @default.
• W2075970918 cites W1973410399 @default.
• W2075970918 cites W1974919845 @default.
• W2075970918 cites W1975030039 @default.
• W2075970918 cites W1977113549 @default.
• W2075970918 cites W1981266474 @default.
• W2075970918 cites W1984726380 @default.
• W2075970918 cites W1985817667 @default.
• W2075970918 cites W1989401199 @default.
• W2075970918 cites W1990449397 @default.
• W2075970918 cites W1990669818 @default.
• W2075970918 cites W1991199914 @default.
• W2075970918 cites W1996338282 @default.
• W2075970918 cites W1997200688 @default.
• W2075970918 cites W2006789066 @default.
• W2075970918 cites W2027121633 @default.
• W2075970918 cites W2028844154 @default.
• W2075970918 cites W2034252391 @default.
• W2075970918 cites W2040353046 @default.
• W2075970918 cites W2049221687 @default.
• W2075970918 cites W2052456123 @default.
• W2075970918 cites W2059679070 @default.
• W2075970918 cites W2063973770 @default.
• W2075970918 cites W2067448597 @default.
• W2075970918 cites W2070073612 @default.
• W2075970918 cites W2078425785 @default.
• W2075970918 cites W2086564003 @default.
• W2075970918 cites W2090933544 @default.
• W2075970918 cites W2092626309 @default.
• W2075970918 cites W2136716105 @default.
• W2075970918 cites W4240258271 @default.
• W2075970918 cites W4242276885 @default.
• W2075970918 cites W4254085062 @default.
• W2075970918 cites W4302346549 @default.
• W2075970918 doi "https://doi.org/10.1006/jmbi.1998.1966" @default.
• W2075970918 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9698558" @default.
• W2075970918 hasPublicationYear "1998" @default.
• W2075970918 type Work @default.
• W2075970918 sameAs 2075970918 @default.
• W2075970918 citedByCount "60" @default.
• W2075970918 countsByYear W20759709182013 @default.
• W2075970918 countsByYear W20759709182016 @default.
• W2075970918 countsByYear W20759709182017 @default.
• W2075970918 countsByYear W20759709182021 @default.
• W2075970918 countsByYear W20759709182023 @default.
• W2075970918 crossrefType "journal-article" @default.
• W2075970918 hasAuthorship W2075970918A5011374485 @default.
• W2075970918 hasAuthorship W2075970918A5021651233 @default.
• W2075970918 hasAuthorship W2075970918A5069818913 @default.
• W2075970918 hasAuthorship W2075970918A5087300006 @default.
• W2075970918 hasConcept C102931765 @default.
• W2075970918 hasConcept C104317684 @default.
• W2075970918 hasConcept C128319531 @default.
• W2075970918 hasConcept C147789679 @default.
• W2075970918 hasConcept C151730666 @default.
• W2075970918 hasConcept C161790260 @default.
• W2075970918 hasConcept C175114707 @default.
• W2075970918 hasConcept C175156509 @default.
• W2075970918 hasConcept C181199279 @default.
• W2075970918 hasConcept C185592680 @default.
• W2075970918 hasConcept C2777028655 @default.
• W2075970918 hasConcept C2778886173 @default.
• W2075970918 hasConcept C2908738455 @default.
• W2075970918 hasConcept C3044715 @default.
• W2075970918 hasConcept C38441620 @default.
• W2075970918 hasConcept C43369102 @default.
• W2075970918 hasConcept C552990157 @default.
• W2075970918 hasConcept C55493867 @default.
• W2075970918 hasConcept C67705224 @default.
• W2075970918 hasConcept C71240020 @default.
• W2075970918 hasConcept C86803240 @default.
• W2075970918 hasConceptScore W2075970918C102931765 @default.
• W2075970918 hasConceptScore W2075970918C104317684 @default.
• W2075970918 hasConceptScore W2075970918C128319531 @default.
• W2075970918 hasConceptScore W2075970918C147789679 @default.
• W2075970918 hasConceptScore W2075970918C151730666 @default.
• W2075970918 hasConceptScore W2075970918C161790260 @default.
• W2075970918 hasConceptScore W2075970918C175114707 @default.
• W2075970918 hasConceptScore W2075970918C175156509 @default.
• W2075970918 hasConceptScore W2075970918C181199279 @default.
• W2075970918 hasConceptScore W2075970918C185592680 @default.
• W2075970918 hasConceptScore W2075970918C2777028655 @default.
• W2075970918 hasConceptScore W2075970918C2778886173 @default.

• next