Matches in SemOpenAlex for { <https://semopenalex.org/work/W2075994511> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2075994511 endingPage "960" @default.
- W2075994511 startingPage "955" @default.
- W2075994511 abstract "A complete data set of the kinetics and some features referring to the substrate specificity and inhibition of UDP-glucose pyrophosphorylase from germinated barley (malt) are given in order to evaluate the use of this easily available enzyme for the synthesis of nucleotide sugars in enzymic glycoconjugate synthesis. In the synthesis direction the Km and Vmax values were 74 μm and 228 U mg−1 for glucose-1-phosphate and 93 μM and 255 U mg−1 for UTP at pH 8.35. UTP gave a substrate surplus inhibition with a KiS of 7.09 mM. For the pyrophosphorylysis reaction the kinetic constants Km and Vmax were 0.191 mM and 350 U mg−1 for UDP-glucose and 0.172 mM and 345 U mg−1 for inorganic pyrophosphate. Inhibition studies demonstrated that UDP-glucose is a competitive inhibitor (Ki 0.117 mM) for UTP and a non-competitive inhibitor (0.015 mM) for glucose-1-phosphate. In the pyrophosphorylysis reaction, UTP is a competitive inhibitor (0.169 mM) for UDP-glucose. Inorganic pyrophosphate (Ki 0.213 and 0.952 mM) and inorganic phosphate (Ki 12.2 and 10.9 mM) were identified as non-competitive inhibitors for glucose-1-phosphate and UTP in the synthesis reaction. The analysis of these inhibition studies revealed a sequential ordered Bi-Bi mechanism. The enzyme is inhibited by free UTP and the optimum ratio of Mg2+/UTP for synthesis of UDP-glucose is between 5 and 10. The enzyme shows relative activities for CTP (18.5%), GTP (14.3%) and ATP (13.7%) when glucose-1-phosphate is the second substrate." @default.
- W2075994511 created "2016-06-24" @default.
- W2075994511 creator A5021636690 @default.
- W2075994511 date "1996-07-01" @default.
- W2075994511 modified "2023-09-26" @default.
- W2075994511 title "Kinetic characterization of UDP-glucose pyrophosphorylase from germinated barley (malt)" @default.
- W2075994511 cites W1486995366 @default.
- W2075994511 cites W1491566690 @default.
- W2075994511 cites W168612836 @default.
- W2075994511 cites W1931599705 @default.
- W2075994511 cites W1992059085 @default.
- W2075994511 cites W1994868945 @default.
- W2075994511 cites W2004626895 @default.
- W2075994511 cites W2010096064 @default.
- W2075994511 cites W2015091013 @default.
- W2075994511 cites W2020422948 @default.
- W2075994511 cites W2027350258 @default.
- W2075994511 cites W2053146826 @default.
- W2075994511 cites W2101629772 @default.
- W2075994511 cites W2140262203 @default.
- W2075994511 cites W4300671741 @default.
- W2075994511 doi "https://doi.org/10.1016/0031-9422(96)00089-1" @default.
- W2075994511 hasPublicationYear "1996" @default.
- W2075994511 type Work @default.
- W2075994511 sameAs 2075994511 @default.
- W2075994511 citedByCount "29" @default.
- W2075994511 countsByYear W20759945112012 @default.
- W2075994511 countsByYear W20759945112013 @default.
- W2075994511 countsByYear W20759945112014 @default.
- W2075994511 countsByYear W20759945112015 @default.
- W2075994511 countsByYear W20759945112017 @default.
- W2075994511 countsByYear W20759945112018 @default.
- W2075994511 countsByYear W20759945112019 @default.
- W2075994511 countsByYear W20759945112021 @default.
- W2075994511 countsByYear W20759945112023 @default.
- W2075994511 crossrefType "journal-article" @default.
- W2075994511 hasAuthorship W2075994511A5021636690 @default.
- W2075994511 hasConcept C104317684 @default.
- W2075994511 hasConcept C118716 @default.
- W2075994511 hasConcept C121332964 @default.
- W2075994511 hasConcept C13965031 @default.
- W2075994511 hasConcept C148898269 @default.
- W2075994511 hasConcept C150757390 @default.
- W2075994511 hasConcept C181199279 @default.
- W2075994511 hasConcept C185592680 @default.
- W2075994511 hasConcept C18903297 @default.
- W2075994511 hasConcept C2776043855 @default.
- W2075994511 hasConcept C2777132085 @default.
- W2075994511 hasConcept C2777289219 @default.
- W2075994511 hasConcept C512185932 @default.
- W2075994511 hasConcept C55493867 @default.
- W2075994511 hasConcept C62520636 @default.
- W2075994511 hasConcept C71240020 @default.
- W2075994511 hasConcept C86803240 @default.
- W2075994511 hasConceptScore W2075994511C104317684 @default.
- W2075994511 hasConceptScore W2075994511C118716 @default.
- W2075994511 hasConceptScore W2075994511C121332964 @default.
- W2075994511 hasConceptScore W2075994511C13965031 @default.
- W2075994511 hasConceptScore W2075994511C148898269 @default.
- W2075994511 hasConceptScore W2075994511C150757390 @default.
- W2075994511 hasConceptScore W2075994511C181199279 @default.
- W2075994511 hasConceptScore W2075994511C185592680 @default.
- W2075994511 hasConceptScore W2075994511C18903297 @default.
- W2075994511 hasConceptScore W2075994511C2776043855 @default.
- W2075994511 hasConceptScore W2075994511C2777132085 @default.
- W2075994511 hasConceptScore W2075994511C2777289219 @default.
- W2075994511 hasConceptScore W2075994511C512185932 @default.
- W2075994511 hasConceptScore W2075994511C55493867 @default.
- W2075994511 hasConceptScore W2075994511C62520636 @default.
- W2075994511 hasConceptScore W2075994511C71240020 @default.
- W2075994511 hasConceptScore W2075994511C86803240 @default.
- W2075994511 hasIssue "4" @default.
- W2075994511 hasLocation W20759945111 @default.
- W2075994511 hasOpenAccess W2075994511 @default.
- W2075994511 hasPrimaryLocation W20759945111 @default.
- W2075994511 hasRelatedWork W1550154378 @default.
- W2075994511 hasRelatedWork W1819654645 @default.
- W2075994511 hasRelatedWork W1972208598 @default.
- W2075994511 hasRelatedWork W2014312418 @default.
- W2075994511 hasRelatedWork W2025788670 @default.
- W2075994511 hasRelatedWork W2029587448 @default.
- W2075994511 hasRelatedWork W2040741921 @default.
- W2075994511 hasRelatedWork W2043126311 @default.
- W2075994511 hasRelatedWork W2090984168 @default.
- W2075994511 hasRelatedWork W2103223904 @default.
- W2075994511 hasVolume "42" @default.
- W2075994511 isParatext "false" @default.
- W2075994511 isRetracted "false" @default.
- W2075994511 magId "2075994511" @default.
- W2075994511 workType "article" @default.