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• W2076003487 abstract "Tgs1 is the enzyme that converts m 7 G RNA caps to the 2,2,7-trimethylguanosine (TMG) caps characteristic of spliceosomal snRNAs. Fungi grow vegetatively without TMG caps, thereby raising the question of what cellular transactions, if any, are TMG cap-dependent. Here, we report that Saccharomyces cerevisiae Tgs1 methyltransferase activity is essential for meiosis. tgs1 Δ cells are specifically defective in splicing PCH2 and SAE3 meiotic pre-mRNAs. The TMG requirement for SAE3 splicing is alleviated by two intron mutations: a UA U UAAC to UA C UAAC change that restores a consensus branchpoint and disruption of a stem–loop encompassing the branchpoint. The TMG requirement for PCH2 splicing is alleviated by a C ACUAAC to U ACUAAC change restoring a consensus branchpoint and by shortening the PCH2 5′ exon. Placing the SAE3 and PCH2 introns within a HIS3 reporter confers Tgs1-dependent histidine prototrophy, signifying that the respective introns are portable determinants of TMG-dependent gene expression. Analysis of in vitro splicing in extracts of TGS1 versus tgs1 Δ cells showed that SAE3 intron removal was enfeebled without TMG caps, whereas splicing of ACT1 was unaffected. Our findings illuminate a new mode of tunable splicing, a reliance on TMG caps for an essential developmental RNA transaction, and three genetically distinct meiotic splicing regulons in budding yeast." @default.
• W2076003487 created "2016-06-24" @default.
• W2076003487 creator A5013910878 @default.
• W2076003487 creator A5014112082 @default.
• W2076003487 creator A5026038727 @default.
• W2076003487 date "2011-03-11" @default.
• W2076003487 modified "2023-10-05" @default.
• W2076003487 title "An essential role for trimethylguanosine RNA caps in Saccharomyces cerevisiae meiosis and their requirement for splicing of SAE3 and PCH2 meiotic pre - mRNAs" @default.
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• W2076003487 doi "https://doi.org/10.1093/nar/gkr083" @default.
• W2076003487 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3141232" @default.
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• W2076003487 hasPublicationYear "2011" @default.
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