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- W2076017382 abstract "It is already known that φX gene A protein converts besides φX RFI DNA also the RFI DNAs of the single-stranded bacteriophages G4, St-1, α3 and φK into RFII DNA. We have extended this observation for bacteriophages G14 and U3. Restriction enzyme analysis placed the φX gene A protein cleavage site in St-1 RF DNA in the Hinf I restriction DNA fragment F 10 and in the overlapping Hae III restriction DNA fragment Z 7 . The exact position and the nucleotide sequence at the 3′-0H end of the nick were determined by DNA sequence analysis of the single-stranded DNA subfragment of the nicked DNA fragment F 10 obtained by gelelectrophoresis in denaturing conditions. A stretch of 85 nucleotdes of St-1 DNA around the position of the φX gene A protein cleavage site was established by DNA sequence analysis of the restriction DNA fragment Z 7 F 1 . Comparison of this nucleotide sequence with the previously determined nucleotide sequence around the cleavage site of φX gene A protein in φX174 RF DNA and G4 RF DNA revealed an identical sequence of only 10 nucleotides. The results suggest that the recognition sequence of the φX174 gene A protein lies within these 10 nucleotides." @default.
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- W2076017382 date "1980-01-01" @default.
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- W2076017382 title "Studies of the recognition sequence of φX174 gene A protein. Cleavage site of φX gene A protein in St-1 RFI DNA" @default.
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- W2076017382 doi "https://doi.org/10.1093/nar/8.9.2009" @default.
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