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- W2076062009 abstract "A major extracellular endoglucanase purified to homogeneity from Thermoascus aurantiacus had a Mr of 34 kDa and a pI of 3.7 and was optimally active at 70–80 °C and pH 4.0–4.4. It was stable at pH 2.8–6.8 at 50 °C for 48 h and maintained its secondary structure and folded conformation up to 70 °C at pH 5.0 and 2.8, respectively. A 33-amino acid sequence at the N terminus showed considerable homology with 14 microbial endoglucanases having highly conserved 8 amino acids (positions 10–17) and Gly, Pro, Gly, and Pro at positions 8, 22, 23, and 32, respectively. The enzyme is rich in Asp (15%) and Glu (10%) with a carbohydrate content of 2.7%. Polyclonal antibodies of endoglucanase cross-reacted with their own antigen and with other purified cellulases from T. aurantiacus. The endoglucanase was specific for polymeric substrates with highest activity toward carboxymethyl cellulose followed by barley β-glucan and lichenan. It preferentially cleaved the internal glycosidic bonds of Glcn and MeUmbGlcn and possessed an extended substrate-binding site with five subsites. The data indicate that the endoglucanase from T. aurantiacus is a member of glycoside hydrolase family 5." @default.
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- W2076062009 date "2002-08-01" @default.
- W2076062009 modified "2023-10-13" @default.
- W2076062009 title "Biochemical characterization and mode of action of a thermostable endoglucanase purified from Thermoascus aurantiacus" @default.
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- W2076062009 doi "https://doi.org/10.1016/s0003-9861(02)00301-6" @default.
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