FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076114460> ?p ?o ?g. }

• W2076114460 endingPage "23572" @default.
• W2076114460 startingPage "23565" @default.
• W2076114460 abstract "Photosystem II uses water as an enzymatic substrate. It has been hypothesized that this water is vectored to the active site for water oxidation via water channels that lead from the surface of the protein complex to the Mn4O5Ca metal cluster. The radiolysis of water by synchrotron radiation produces amino acid residue-modifying OH• and is a powerful technique to identify regions of proteins that are in contact with water. In this study, we have used this technique to oxidatively modify buried amino acid residues in higher plant Photosystem II membranes. Fourier transform ion cyclotron resonance mass spectrometry was then used to identify these oxidized amino acid residues that were located in several core Photosystem II subunits (D1, D2, CP43, and CP47). While, as expected, the majority of the identified oxidized residues (≈75%) are located on the solvent-exposed surface of the complex, a number of buried residues on these proteins were also modified. These residues form groups which appear to lead from the surface of the complex to the Mn4O5Ca cluster. These residues may be in contact with putative water channels in the photosystem. These results are discussed within the context of a number of largely computational studies that have identified putative water channels in Photosystem II.Background: Substrate water must reach the buried Mn4O5Ca cluster in Photosystem II.Results: OH• produced by radiolysis modified buried amino acid residues. These were mapped onto the PS II crystal structure.Conclusion: Two groups of oxidized residues were identified which form putative pathways to the Mn4O5Ca cluster.Significance: Identification of water and oxygen channels is crucial for our understanding of Photosystem II function. Photosystem II uses water as an enzymatic substrate. It has been hypothesized that this water is vectored to the active site for water oxidation via water channels that lead from the surface of the protein complex to the Mn4O5Ca metal cluster. The radiolysis of water by synchrotron radiation produces amino acid residue-modifying OH• and is a powerful technique to identify regions of proteins that are in contact with water. In this study, we have used this technique to oxidatively modify buried amino acid residues in higher plant Photosystem II membranes. Fourier transform ion cyclotron resonance mass spectrometry was then used to identify these oxidized amino acid residues that were located in several core Photosystem II subunits (D1, D2, CP43, and CP47). While, as expected, the majority of the identified oxidized residues (≈75%) are located on the solvent-exposed surface of the complex, a number of buried residues on these proteins were also modified. These residues form groups which appear to lead from the surface of the complex to the Mn4O5Ca cluster. These residues may be in contact with putative water channels in the photosystem. These results are discussed within the context of a number of largely computational studies that have identified putative water channels in Photosystem II. Background: Substrate water must reach the buried Mn4O5Ca cluster in Photosystem II. Results: OH• produced by radiolysis modified buried amino acid residues. These were mapped onto the PS II crystal structure. Conclusion: Two groups of oxidized residues were identified which form putative pathways to the Mn4O5Ca cluster. Significance: Identification of water and oxygen channels is crucial for our understanding of Photosystem II function." @default.
• W2076114460 created "2016-06-24" @default.
• W2076114460 creator A5005259914 @default.
• W2076114460 creator A5013659856 @default.
• W2076114460 creator A5026390148 @default.
• W2076114460 creator A5037885044 @default.
• W2076114460 creator A5081507685 @default.
• W2076114460 creator A5088111642 @default.
• W2076114460 date "2013-08-01" @default.
• W2076114460 modified "2023-10-03" @default.
• W2076114460 title "Radiolytic Mapping of Solvent-Contact Surfaces in Photosystem II of Higher Plants" @default.
• W2076114460 cites W1558317964 @default.
• W2076114460 cites W1961714136 @default.
• W2076114460 cites W1964153219 @default.
• W2076114460 cites W1966485177 @default.
• W2076114460 cites W1975032814 @default.
• W2076114460 cites W1976352074 @default.
• W2076114460 cites W1981593008 @default.
• W2076114460 cites W1981892500 @default.
• W2076114460 cites W1982351418 @default.
• W2076114460 cites W1982902126 @default.
• W2076114460 cites W1983684702 @default.
• W2076114460 cites W1994641074 @default.
• W2076114460 cites W1998345494 @default.
• W2076114460 cites W1999128746 @default.
• W2076114460 cites W1999904181 @default.
• W2076114460 cites W2000390034 @default.
• W2076114460 cites W2005594958 @default.
• W2076114460 cites W2005727820 @default.
• W2076114460 cites W2008881978 @default.
• W2076114460 cites W2010289530 @default.
• W2076114460 cites W2011152207 @default.
• W2076114460 cites W2026127059 @default.
• W2076114460 cites W2036724345 @default.
• W2076114460 cites W2039616447 @default.
• W2076114460 cites W2045743530 @default.
• W2076114460 cites W2047903321 @default.
• W2076114460 cites W2052727753 @default.
• W2076114460 cites W2057834652 @default.
• W2076114460 cites W2057863409 @default.
• W2076114460 cites W2068374596 @default.
• W2076114460 cites W2070675485 @default.
• W2076114460 cites W2079213338 @default.
• W2076114460 cites W2080418501 @default.
• W2076114460 cites W2082121100 @default.
• W2076114460 cites W2083907491 @default.
• W2076114460 cites W2084032366 @default.
• W2076114460 cites W2085709476 @default.
• W2076114460 cites W2091502101 @default.
• W2076114460 cites W2105125469 @default.
• W2076114460 cites W2127143218 @default.
• W2076114460 cites W2132251312 @default.
• W2076114460 cites W2135433724 @default.
• W2076114460 cites W2142184469 @default.
• W2076114460 cites W2160704158 @default.
• W2076114460 cites W2171093931 @default.
• W2076114460 cites W2243263300 @default.
• W2076114460 doi "https://doi.org/10.1074/jbc.m113.487033" @default.
• W2076114460 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3949330" @default.
• W2076114460 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23814046" @default.
• W2076114460 hasPublicationYear "2013" @default.
• W2076114460 type Work @default.
• W2076114460 sameAs 2076114460 @default.
• W2076114460 citedByCount "25" @default.
• W2076114460 countsByYear W20761144602014 @default.
• W2076114460 countsByYear W20761144602015 @default.
• W2076114460 countsByYear W20761144602016 @default.
• W2076114460 countsByYear W20761144602017 @default.
• W2076114460 countsByYear W20761144602018 @default.
• W2076114460 countsByYear W20761144602019 @default.
• W2076114460 countsByYear W20761144602020 @default.
• W2076114460 countsByYear W20761144602022 @default.
• W2076114460 countsByYear W20761144602023 @default.
• W2076114460 crossrefType "journal-article" @default.
• W2076114460 hasAuthorship W2076114460A5005259914 @default.
• W2076114460 hasAuthorship W2076114460A5013659856 @default.
• W2076114460 hasAuthorship W2076114460A5026390148 @default.
• W2076114460 hasAuthorship W2076114460A5037885044 @default.
• W2076114460 hasAuthorship W2076114460A5081507685 @default.
• W2076114460 hasAuthorship W2076114460A5088111642 @default.
• W2076114460 hasBestOaLocation W20761144601 @default.
• W2076114460 hasConcept C139066938 @default.
• W2076114460 hasConcept C154828652 @default.
• W2076114460 hasConcept C183688256 @default.
• W2076114460 hasConcept C185592680 @default.
• W2076114460 hasConcept C515207424 @default.
• W2076114460 hasConcept C55493867 @default.
• W2076114460 hasConcept C66887884 @default.
• W2076114460 hasConcept C75473681 @default.
• W2076114460 hasConcept C80298142 @default.
• W2076114460 hasConceptScore W2076114460C139066938 @default.
• W2076114460 hasConceptScore W2076114460C154828652 @default.
• W2076114460 hasConceptScore W2076114460C183688256 @default.
• W2076114460 hasConceptScore W2076114460C185592680 @default.
• W2076114460 hasConceptScore W2076114460C515207424 @default.
• W2076114460 hasConceptScore W2076114460C55493867 @default.
• W2076114460 hasConceptScore W2076114460C66887884 @default.
• W2076114460 hasConceptScore W2076114460C75473681 @default.

• next