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- W2076128220 abstract "The fourescence associated with benzo(a)pyrene (BP) moietes covalently attached to the nucleic acid (DNA plus RNA) isolated from the epidermis of BP-treated mice was examined at 77 K in frozen aqueous solution by use of a photon-counting fluorimeter operating in the synchronous scanning mode. The excitation and emmission wavelenghts were scanned simultaneously with the monochromators set 28 nm apart. This setting coincides with the difference in wavelength between the excitation and emission maxima for the fluorescence of bound BP. Currently the level of detection is in the order of 1 BP residue from the skin of a single mouse. The method described here is generally useful for detecting the binding to DNA of nonradioactive carcinogic polynuclear aromatic which might occur following the topical application to animal skin in vivo complex hydrocarbon mixtures such as synthetic and crude oils." @default.
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- W2076128220 date "1980-11-01" @default.
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- W2076128220 title "Binding on benzo (a) pyrene to epidermal DNA and RNA as detected by synchronous luminescence spectrometry at 77 K" @default.
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- W2076128220 doi "https://doi.org/10.1016/0165-022x(80)90009-3" @default.
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